PMID: 11923101Mar 30, 2002Paper

Both isoforms of mammalian phosphatidylinositol transfer protein are capable of binding and transporting sphingomyelin

Biochimica Et Biophysica Acta
Hong LiGeorge M Helmkamp

Abstract

The structurally related mammalian alpha and beta isoforms of phosphatidylinositol (PtdIns) transfer protein (PITP) bind reversibly a single phospholipid molecule, preferably PtdIns or phosphatidylcholine (PtdCho), and transport that lipid between membrane surfaces. PITPbeta, but not PITPalpha, is reported extensively in the scientific literature to exhibit the additional capacity to bind and transport sphingomyelin (CerPCho). We undertook a detailed investigation of the lipid binding and transfer specificity of the soluble mammalian PITP isoforms. We employed a variety of donor and acceptor membrane lipid compositions to determine the sensitivity of recombinant rat PITPalpha and PITPbeta isoforms toward PtdIns, PtdCho, CerPCho, and phosphatidate (PtdOH). Results indicated often striking differences in protein-phospholipid and protein-membrane interactions. We demonstrated unequivocally that both isoforms were capable of binding and transferring CerPCho; we confirmed that the beta isoform was the more active. The order of transfer specific activity was similar for both isoforms: PtdIns>PtdCho>CerPCho>PtdOH. Independently, we verified the binding of CerPCho to both isoforms by showing an increase in holoprotein isoelectric point...Continue Reading

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Citations

May 24, 2005·Biochimica Et Biophysica Acta·Ryan J Perry, Neale D Ridgway
Sep 18, 2013·The Journal of Biological Chemistry·Christiaan van OoijMichael J Blackman
Oct 26, 2016·Chemistry and Physics of Lipids·Mark I McDermott, Carl J Mousley
Oct 2, 2007·Journal of Lipid Research·Per HaberkantBritta Brügger
Oct 29, 2002·FEBS Letters·Victoria Allen-BaumeShamshad Cockcroft
Sep 9, 2010·Nature Reviews. Molecular Cell Biology·Sima Lev
Apr 14, 2016·Biochemical Society Transactions·Michael Selitrennik, Sima Lev
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Diego PerettiSima Lev
Nov 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Atsuko K Kimura, Tomohiro Kimura
May 11, 2007·Biochimica Et Biophysica Acta·Shamshad Cockcroft, Nicolas Carvou

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