Both LyF-1 and an Ets protein interact with a critical promoter element in the murine terminal transferase gene.

Molecular and Cellular Biology
P ErnstS T Smale

Abstract

Terminal deoxynucleotidyltransferase (TdT) is a template-independent DNA polymerase that is expressed transiently during the earliest stages of B- and T-cell ontogeny. Previously, we characterized the promoter for the murine TdT gene and identified a novel DNA-binding protein, called LyF-1, that interacts with a DNA sequence element found to be critical for transcriptional activity in lymphoid cell lines. Here, we present a more detailed analysis of this 30-bp control element, called the TdT D' element, which is centered approximately 60 bp upstream of the transcription start site. We found that both the murine and human D' elements are recognized by multiple proteins, including LyF-1 and at least two Ets family proteins, Ets-1 and Fli-1. Additional protein-DNA interactions were identified through studies using unfractionated nuclear extracts, in which the D' element was apparently incorporated into a multiprotein complex, possibly containing an Ets protein as a core component. By analyzing a series of substitution mutations, two adjacent binding sites for LyF-1 were identified in the murine D' element, with the Ets protein binding site closely coinciding with the proximal, lower-affinity LyF-1 site. Transient transfection anal...Continue Reading

References

Jan 1, 1992·Annual Review of Immunology·K IkutaI L Weissman
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J Hagman, R Grosschedl
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KallenbachF Rougeon
Jun 1, 1992·Molecular and Cellular Biology·J M AllenR M Perlmutter
Jan 1, 1992·Annual Review of Immunology·L H Glimcher, C J Kara
Jun 1, 1992·Genes & Development·C WasylykB Wasylyk
Jan 1, 1992·Molecular and Cellular Biology·J M PongubalaM L Atchison
Dec 20, 1991·Cell·D B Starr, D K Hawley
Jan 1, 1991·Annual Review of Immunology·L M Staudt, M J Lenardo
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L K RileyM S Coleman
Jan 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D P WirtJ M Layton
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H Martinez-Valdez, A Cohen
Nov 20, 1987·Science·F W AltG D Yancopoulos
Jul 15, 1988·Biochemical and Biophysical Research Communications·O Koiwai, A Morita
Jul 2, 1971·Biochemical and Biophysical Research Communications·L M Chang

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Citations

Feb 15, 2001·Oncogene·V I Sementchenko, D K Watson
Oct 1, 1994·Molecular Reproduction and Development·M J Tymms, I Kola
Jan 29, 2008·The Journal of Biological Chemistry·Zafer GurelSinisa Dovat
Oct 18, 1996·The Journal of Biological Chemistry·Y AkbaraliT A Libermann
Sep 26, 2000·Molecular and Cellular Biology·D W O'NeillA Bank
Jan 1, 2009·Médecine sciences : M/S·Marilyne DijonCécile Tonnelle
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C WeiI Goldschneider
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kimberly J PayneGay M Crooks
Mar 15, 2002·Current Opinion in Genetics & Development·Amanda G Fisher, Matthias Merkenschlager
Nov 1, 1996·Molecular and Cellular Biology·P ErnstS T Smale
Sep 6, 2003·Gene·Marta AlvarezJoseph P Bidwell
Apr 1, 1994·Current Opinion in Immunology·J Hagman, R Grosschedl
Mar 2, 2005·Seminars in Immunology·Ruey-Chyi SuStephen T Smale

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