PMID: 8997199Dec 1, 1996Paper

Both M1 and M3 receptors regulate exocrine secretion by mucous acini

The American Journal of Physiology
D J CulpL R Latchney

Abstract

We investigated the role of M1 and M3 receptors in regulating exocrine secretion from acini isolated from rat sublingual glands. In secretion experiments, we derived affinity values (KB) from Schild regression analysis for the antagonists pirenzepine (61.0 nM) and 4-diphenylacetoxy-N-methylpiperidine (4-DAMP; 1.06 nM). The KB for 4-DAMP is similar to its affinity value [equilibrium dissociation constant from competition studies (Ki); 1.81 nM] determined from radioligand competition experiments. In contrast, the KB for pirenzepine is between its high-affinity (17.6 nM) and low-affinity (404 nM) Ki values. In separate secretion experiments, we found that the M1 receptor antagonist, M1-toxin, induces a rightward shift in the concentration-response curve to muscarinic agonist and inhibits maximal secretion by 40%. The inhibitory effect of M1-toxin appears specific for M1 receptor blockade, since the toxin abolishes acinar high-affinity pirenzepine-binding sites and does not inhibit secretion induced by nonmuscarinic agents. Additional pharmacological studies indicate muscarinic receptors do not function through putative neural elements within isolated acini. Our combined results are consistent with both M1 and M3 receptors directly...Continue Reading

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Citations

Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J E Melvin
Mar 17, 2006·Journal of Applied Physiology·Gordon B Proctor
Oct 28, 2005·Caries Research·D J CulpR Faustoferri
Nov 19, 2003·Current Urology Reports·Harriette M Scarpero, Roger R Dmochowski
Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Mar 14, 2001·American Journal of Physiology. Cell Physiology·W LuoD J Culp
Dec 3, 2005·Journal of Applied Physiology·G TobinA V Edwards
Sep 22, 2011·Journal of Dental Research·D J CulpR L Evans
Oct 15, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Biswadip DasDavid J Culp
Apr 13, 2013·The Journal of Biological Chemistry·Biswadip DasDavid J Culp
Feb 12, 2019·Archives of Oral Biology·Atsara RawangwongWiphawi Hipkaeo
Dec 1, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·R I FoxA Fisher
Nov 9, 2002·Autonomic Neuroscience : Basic & Clinical·Gunnar TobinBengt Götrick
Dec 13, 2006·Autonomic Neuroscience : Basic & Clinical·Gordon B Proctor, Guy H Carpenter
Sep 11, 2007·Archives of Oral Biology·Anders T RybergGunnar Tobin

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