Both maltose-binding protein and ATP are required for nucleotide-binding domain closure in the intact maltose ABC transporter.

Proceedings of the National Academy of Sciences of the United States of America
Cédric OrelleAmy L Davidson

Abstract

The maltose transporter MalFGK(2) of Escherichia coli is a member of the ATP-binding cassette superfamily. A periplasmic maltose-binding protein (MBP) delivers maltose to MalFGK(2) and stimulates its ATPase activity. Site-directed spin labeling EPR spectroscopy was used to study the opening and closing of the nucleotide-binding interface of MalFGK(2) during the catalytic cycle. In the intact transporter, closure of the interface coincides not just with the binding of ATP, as seen with isolated nucleotide-binding domains, but requires both MBP and ATP, implying that MBP stimulates ATPase activity by promoting the closure of the nucleotide-binding interface. After ATP hydrolysis, with MgADP and MBP bound, the nucleotide-binding interface resides in a semi-open configuration distinct from the fully open configuration seen in the absence of any ligand. We propose that P(i) release coincides with the reorientation of transmembrane helices to an inward-facing conformation and the final step of maltose translocation into the cell.

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Citations

Dec 5, 2008·Biochemistry·Kathryn M WestfahlCandice S Klug
Jun 29, 2010·Journal of the American Chemical Society·Frances Joan D AlvarezAmy L Davidson
Feb 17, 2009·Nature Chemical Biology·Enrico Schleiff, Robert Tampé
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Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael L Oldham, Jue Chen
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