Both mitogen-activated protein kinase and phosphatidylinositol 3-kinase signalling are required in epidermal growth factor-induced human trophoblast migration

Molecular Human Reproduction
Qing QiuAndrée Gruslin

Abstract

Adequate extravillous trophoblast (EVT) invasion is an essential step for placental formation. The aim of this study was to examine the possible role of phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinase (MAPK) signalling in epidermal growth factor (EGF)-induced EVT migration and to determine if the 70 kDa ribosomal S6 kinase (p70S6K) is involved in this process. In this study, EGF significantly stimulated HTR8/SVneo cell migration and the phosphorylation of AKT, ERK1/2 and p70S6K in a concentration-dependent manner. The MAPK inhibitor U0126 decreased cell migration and ERK phosphorylation, but it did not influence p70S6K phosphorylation in response to EGF. In the presence of PI3K inhibitors (Wortmannin), EGF-stimulated trophoblast migration and phosphorylation of AKT and P70S6K (Thr(389) and Thr(421)/Ser(424)) were decreased, while EGF-induced ERK phosphorylation was not affected. Expression of an activated AKT (Myr-AKT2) increased basal phospho-p70S6K (Thr(389) and Thr(421)/Ser(424)) content, but failed to stimulate cell migration. However, it induced cell migration in the presence of EGF and Wortmannin, in which both AKT and MAPK pathways were activated. In addition, there was a concentration-dependent...Continue Reading

Citations

Jan 25, 2008·Cancer Chemotherapy and Pharmacology·Chao JiYinsheng Wan
Sep 10, 2013·Human Reproduction·Cheuk-Lun LeePhilip C N Chiu
Mar 4, 2005·Biology of Reproduction·Arlen SoghomoniansGordon C Douglas
Nov 29, 2008·Biology of Reproduction·L C SchulzR M Roberts
Jan 10, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Zhihui TanYu Zhang
Sep 29, 2011·Molecular and Cellular Biology·Lindsey N KentMichael J Soares
Jul 14, 2012·The Journal of Reproduction and Development·Michael J SoaresM A Karim Rumi
Oct 31, 2009·The International Journal of Developmental Biology·Martin Knöfler
May 9, 2012·The International Journal of Developmental Biology·Lindsey N KentMichael J Soares
Jan 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Simone CodeluppiElena B Pasquale
Jul 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qing QiuAndrée Gruslin
Nov 22, 2007·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Vincent L Morris, Bosco M C Chan
Jul 7, 2011·Journal of Cellular Physiology·Pasquapina CiarmelaMario Castellucci
Apr 13, 2011·American Journal of Reproductive Immunology : AJRI·Angela R Lemons, Rajesh K Naz
Apr 20, 2005·Placenta·M KnöflerS Hauguel-de Mouzon
Apr 14, 2016·The Journal of Pathology·Fu-Ju TianYi Lin
Oct 6, 2009·American Journal of Obstetrics and Gynecology·Juan A ArroyoHenry L Galan

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