Both Nuclear Size and DNA Amount Contribute to Midblastula Transition Timing in Xenopus laevis

Scientific Reports
Predrag Jevtić, Daniel L Levy

Abstract

During early Xenopus laevis embryogenesis both nuclear and cell volumes decrease with the nuclear-to-cytoplasmic (N/C) volume ratio reaching a maximum at the midblastula transition (MBT). At the MBT, embryonic transcription is upregulated and cell cycles lengthen. Early studies demonstrated a role for the DNA-to-cytoplasmic ratio in the control of MBT timing. By altering nuclear size, we previously showed that the N/C volume ratio also contributes to proper MBT timing. Here we examine the relative contributions of nuclear size and DNA amount to MBT timing by simultaneously altering nuclear size and ploidy in X. laevis embryos. Compared to diploid embryos, haploids exhibited a delay in both zygotic gene expression and cell cycle lengthening, while diploid embryos with increased N/C volume ratios showed early expression of zygotic genes and premature lengthening of cell cycles. Interestingly, haploids with increased N/C volume ratios exhibited an intermediate effect on the timing of zygotic gene expression and cell cycle lengthening. Decreasing nuclear size in post-MBT haploid embryos caused a further delay in cell cycle lengthening and the expression of some zygotic genes. Our data suggest that both the N/C volume ratio and DNA ...Continue Reading

References

Jul 1, 1984·Cell·J W Newport, Marc W Kirschner
Oct 16, 2010·Cell·Daniel L Levy, Rebecca Heald
Feb 25, 2011·PloS One·Livia VastagMarc W Kirschner
Aug 30, 2013·The Journal of Biological Chemistry·Christopher M Murphy, W Matthew Michael
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Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Amanda A AmodeoJan M Skotheim

Citations

Nov 6, 2018·The FEBS Journal·Aline Xavier da Silveira Dos Santos, Prisca Liberali
Feb 1, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Chase C WesleyDaniel L Levy
Jun 28, 2020·Journal of Cell Science·Yuki Hara
Jun 14, 2019·Development·Nadine L VastenhouwHoward D Lipshitz
Dec 23, 2018·Nature Reviews. Genetics·Katharine N Schulz, Melissa M Harrison
Oct 1, 2020·Molecular Biology of the Cell·Hiroko HeijoYuki Hara
Apr 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Sahla SyedAmanda A Amodeo

Methods Mentioned

BETA
PCR

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