PMID: 11911364Mar 26, 2002Paper

Both ran and importins have the ability to function as nuclear mRNA export factors

RNA
Rui YiB R Cullen

Abstract

The Ran protein regulates nucleocytoplasmic transport mediated by the karyopherin family of nuclear transport factors. Ran is converted to the active, GTP bound form in the nucleus and then binds to a conserved domain found in all karyopherins. This interaction induces cargo binding for exportins and cargo release for importins. In either case, the Ran.GTP is then transported to the cytoplasm by the karyopherin, where it is hydrolyzed to Ran.GDP. To ask whether Ran could function as a nuclear mRNA export factor, we fused Ran to the MS2 coat protein and inserted MS2 RNA-binding sites into an unspliced cat mRNA that is normally sequestered in the nucleus. Coexpression of MS2-Ran induced cat mRNA export and CAT enzyme expression as effectively as, for example, an MS2-Rev fusion protein. MS2-Ran dependent nuclear mRNA export was reduced by inhibitors specific for Crm1, but not blocked as was seen with MS2-Rev. Consistent with the hypothesis that Crm1 is not the only karyopherin cofactor for MS2-Ran mediated mRNA export, we show that not only Crm1 but also CAS, transportin, importin beta and exportin t can all export mRNA from the nucleus when tethered via the MS2 RNA-binding domain. In contrast, two shuttling hnRNPs, hnRNP A1 and h...Continue Reading

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Citations

Oct 23, 2004·Best Practice & Research. Clinical Haematology·Michel SadelainIsabelle Rivière
Dec 19, 2003·Genes & Development·Rui YiBryan R Cullen
Nov 18, 2011·BMC Molecular Biology·Takashi MikiYoshihiro Yoneda
Jan 22, 2009·RNA·Kelly D SullivanEric J Wagner
Apr 2, 2003·The Journal of Cell Biology·Frederic KendirgiSusan R Wente
Dec 13, 2005·Annals of the New York Academy of Sciences·Michel SadelainIsabelle Riviere
Dec 12, 2003·The Journal of Biological Chemistry·Armelle RoisinPierre Jalinot

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