Both the autophagy and proteasomal pathways facilitate the Ubp3p-dependent depletion of a subset of translation and RNA turnover factors during nitrogen starvation in Saccharomyces cerevisiae

RNA
Shane P Kelly, David M Bedwell

Abstract

Protein turnover is an important regulatory mechanism that facilitates cellular adaptation to changing environmental conditions. Previous studies have shown that ribosome abundance is reduced during nitrogen starvation by a selective autophagy mechanism termed ribophagy, which is dependent upon the deubiquitinase Ubp3p. In this study, we asked whether the abundance of various translation and RNA turnover factors are reduced following the onset of nitrogen starvation in Saccharomyces cerevisiae. We found distinct differences in the abundance of the proteins tested following nitrogen starvation: (1) The level of some did not change; (2) others were reduced with kinetics similar to ribophagy, and (3) a few proteins were rapidly depleted. Furthermore, different pathways differentially degraded the various proteins upon nitrogen starvation. The translation factors eRF3 and eIF4GI, and the decapping enhancer Pat1p, required an intact autophagy pathway for their depletion. In contrast, the deadenylase subunit Pop2p and the decapping enzyme Dcp2p were rapidly depleted by a proteasome-dependent mechanism. The proteasome-dependent depletion of Dcp2p and Pop2p was also induced by rapamycin, suggesting that the TOR1 pathway influences this...Continue Reading

References

Mar 28, 1998·The Journal of Biological Chemistry·T Noda, Y Ohsumi
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C BersetM Altmann
Nov 1, 2000·Cell·T Schmelzle, M N Hall
Nov 15, 2000·Biological Chemistry·A Y AmerikM Hochstrasser
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·T F ChanX F Zheng
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·F G KuruvillaS L Schreiber
Jun 5, 2003·Nature Cell Biology·Mickaël CohenCatherine Dargemont
Feb 6, 2004·The Journal of Biological Chemistry·Jun Onodera, Yoshinori Ohsumi
Aug 18, 2004·Molecular and Cellular Biology·Joe Salas-Marco, David M Bedwell
Dec 2, 2004·Biochimica Et Biophysica Acta·Michael Rape, Stefan Jentsch
Jul 20, 2005·The Journal of Biological Chemistry·Jun Onodera, Yoshinori Ohsumi
Dec 24, 2005·Science·Ramunas M Vabulas, F Ulrich Hartl
Oct 6, 2006·Molecular Biology of the Cell·Zhifen YangDaniel J Klionsky
Jan 6, 2007·Autophagy·Zhifen Yang, Daniel J Klionsky
Jul 17, 2007·Journal of Molecular Biology·Keqin LiRonen Marmorstein
Apr 23, 2008·The Journal of Cell Biology·Francisco Ramírez-ValleRobert J Schneider
Jun 2, 2009·Biological & Pharmaceutical Bulletin·Hye Kyoung JinJeung-Whan Han
Dec 10, 2009·Molecular and Cellular Biology·Yoshiaki KamadaYoshinori Ohsumi
Feb 12, 2010·FEBS Letters·Melinda A Lynch-Day, Daniel J Klionsky
Feb 18, 2010·PloS One·Bryan K ClarksonJennifer A Doudna
May 29, 2010·EMBO Reports·Batool Ossareh-NazariCatherine Dargemont
Sep 3, 2010·Nature Cell Biology·Claudine KraftKay Hofmann
Sep 22, 2010·The Journal of Cell Biology·Roswitha KrickMichael Thumm
Sep 30, 2010·The Journal of Biological Chemistry·Peng Mao, Michael J Smerdon
Aug 3, 2011·Biochimica Et Biophysica Acta·Catherine Dargemont, Batool Ossareh-Nazari
Dec 25, 2013·Cell Research·Yuchen FengDaniel J Klionsky
Mar 13, 2014·The Journal of Cell Biology·Batool Ossareh-NazariCatherine Dargemont

❮ Previous
Next ❯

Citations

Oct 8, 2016·Autophagy·Lisa B FrankelAnders H Lund
Aug 16, 2018·Genes & Development·Alexandra ZinovievTatyana V Pestova
Apr 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Shinju Sugiyama, Motomasa Tanaka
May 30, 2019·Molecular Biology of the Cell·Peyman P AryanpurTimothy A Bolger
Jun 10, 2017·The EMBO Journal·Lorenzo GalluzziGuido Kroemer
Nov 20, 2019·Nature Plants·Toshifumi Inada
Jul 24, 2015·Applied Microbiology and Biotechnology·Catherine TesnièreBruno Blondin
Nov 4, 2016·Genes & Development·Stacie E DodgsonAngelika Amon
Dec 19, 2018·Wiley Interdisciplinary Reviews. RNA·Elizabeth Delorme-Axford, Daniel J Klionsky
Sep 23, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jingqi YanR Suzanne Zukin
Oct 22, 2020·Open Biology·Harsha Garadi SureshBrenda Andrews
Mar 5, 2021·The FEBS Journal·Fivos Borbolis, Popi Syntichaki

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.