Both the dimerization and immunochemical properties of E-cadherin EC1 domain depend on Trp(156) residue

Archives of Biochemistry and Biophysics
O Y LaurS Troyanovsky

Abstract

Using site-directed mutagenesis, we show in this paper that the adhesive interface detected in cadherin crystals is unlikely to mediate adhesive interaction between myc- and flag-tagged E-cadherin molecules in human A-431 cells. We also found that a critical residue within this interface, His(233), is part of the epitope for mAb SHE78-7. This epitope was accessible to the antibody in the adhesive E-cadherin dimers, which is consistent with uninvolvement of the site containing His(233) in cell-cell adhesion. However, both the adhesive dimerization and the integrity of the SHE78-7 epitope depended on the same intramolecular interaction between Trp(156) and its hydrophobic pocket. Our data suggest that this interaction may have an important regulatory function in controlling the surface topology of the NH(2)-terminal domain of E-cadherin.

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Citations

Mar 2, 2010·Nature Structural & Molecular Biology·Oliver J HarrisonLawrence Shapiro
Jun 9, 2006·Molecular Biology of the Cell·Regina B TroyanovskySergey M Troyanovsky
Sep 1, 2007·Molecular Biology of the Cell·Regina B TroyanovskySergey M Troyanovsky
Jul 18, 2008·Molecular Biology of the Cell·Alexi KissSergey M Troyanovsky
Oct 31, 2003·Molecular and Cellular Biology·Regina B TroyanovskySergey M Troyanovsky
Jul 13, 2006·Annual Review of Biomedical Engineering·Deborah Leckband, Anil Prakasam
Jun 2, 2007·Annual Review of Cell and Developmental Biology·Sabine Pokutta, William I Weis
Jul 31, 2010·Dermatology Research and Practice·Gethin R Owen, David L Stokes
Mar 1, 2012·Journal of Cell Science·Fei GengDavid W Andrews
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Barry M Gumbiner
Mar 23, 2011·The Journal of Cell Biology·Soonjin HongSergey M Troyanovsky
Apr 3, 2013·The Journal of Cell Biology·Soonjin HongSergey M Troyanovsky
Aug 4, 2004·Cellular and Molecular Life Sciences : CMLS·A W KochW Shan
Apr 12, 2005·European Journal of Cell Biology·Sergey Troyanovsky
Feb 28, 2003·Oncogene·Jörg KlingelhöferSergey Troyanovsky
Jan 27, 2005·Journal of Cell Science·Oliver J HarrisonPeter J Kilshaw
Jul 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Regina B TroyanovskySergey M Troyanovsky

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