PMID: 11340188May 8, 2001Paper

Both the extracellular leucine-rich repeat domain and the kinase activity of FSL2 are required for flagellin binding and signaling in Arabidopsis

The Plant Cell
L Gómez-GómezThomas Boller

Abstract

In Arabidopsis, activation of defense responses by flagellin is triggered by the specific recognition of the most conserved domain of flagellin, represented by the peptide flg22, in a process involving the FLS2 gene, which encodes a leucine-rich repeat serine/threonine protein kinase. We show here that the two fls2 mutant alleles, fls2-24 and fls2-17, which were shown previously to confer insensitivity to flg22, also cause impaired flagellin binding. These features are rescued when a functional FLS2 gene is expressed as a transgene in each of the fls2 mutant plants, indicating that FLS2 is necessary for flagellin binding. The point mutation of the fls2-17 allele lies in the kinase domain. A kinase carrying this missense mutation lacked autophosphorylation activity when expressed in Escherichia coli. This indicates that kinase activity is required for binding and probably affects the stability of the flagellin receptor complex. We further show that overexpression of the kinase-associated protein phosphatase (KAPP) in Arabidopsis results in plants that are insensitive to flagellin treatment, and we show reduced flg22 binding in these plants. Furthermore, using the yeast two-hybrid system, we show physical interaction of KAPP with...Continue Reading

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Dec 1, 1994·Current Opinion in Structural Biology·E J Goldsmith, M H Cobb
Oct 1, 1994·Trends in Biochemical Sciences·B Kobe, J Deisenhofer
Jul 1, 1997·Genes & Development·Y YangD F Klessig
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·R W WilliamsE M Meyerowitz
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·L Gómez-GómezT Boller
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·J LiJ C Walker
Mar 14, 2000·The Journal of Biological Chemistry·T S NühseT Boller
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J LiuJ K Zhu
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J L GirantonT Gaude
Jun 30, 2000·Current Opinion in Plant Biology·J EllisT Pryor
Aug 1, 2000·Science·A E TrotochaudS E Clark
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Oct 6, 2000·Current Opinion in Plant Biology·K U Torii

❮ Previous
Next ❯

Citations

Jan 1, 2002·The Arabidopsis Book·Fumiaki KatagiriSheng Yang He
Oct 12, 2012·Protoplasma·Silke Robatzek, Lennart Wirthmueller
May 11, 2013·Journal of Biosciences·Mehanathan Muthamilarasan, Manoj Prasad
Feb 23, 2002·Trends in Genetics : TIG·Noni V E Franklin-Tong
Mar 14, 2002·Current Opinion in Cell Biology·J Mark CockThierry Gaude
Aug 10, 2001·Trends in Plant Science·T Nürnberger, D Scheel
Jun 7, 2002·Trends in Plant Science·Lourdes Gómez-Gómez, Thomas Boller
May 22, 2003·Trends in Plant Science·Gabrielle TichtinskyThierry Gaude
Oct 13, 2001·Current Opinion in Plant Biology·T Romeis
Aug 16, 2002·Current Opinion in Plant Biology·Giulia De Lorenzo, Simone Ferrari
Aug 17, 2002·Current Opinion in Plant Biology·Seiji Takayama, Youji Sakagami
Aug 17, 2002·Current Opinion in Plant Biology·Colin Brownlee
Jul 23, 2003·Current Opinion in Plant Biology·Erin R Morris, John C Walker
Sep 16, 2003·Current Opinion in Plant Biology·Anne Diévart, Steven E Clark
Aug 1, 2002·The Plant Journal : for Cell and Molecular Biology·Anthony P Fordham-SkeltonJohn A Gatehouse
Mar 19, 2002·Cellular Microbiology·Arnaud DidierlaurentMarian R Neutra
Apr 22, 2011·Proceedings of the National Academy of Sciences of the United States of America·Aizhong CaoPeidu Chen
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Cristian H DannaFrederick M Ausubel
Jul 4, 2009·Journal of Experimental Botany·Lindsay N PetersenKatherine J Denby
Nov 6, 2010·Plant & Cell Physiology·Hidenori MatsuiHirohiko Hirochika
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Xiangdong MengSteven V Beer
Sep 14, 2007·Molecular Plant-microbe Interactions : MPMI·Denise Altenbach, Silke Robatzek
Nov 7, 2008·Molecular Plant-microbe Interactions : MPMI·Leendert C van LoonAlain Pugin
Feb 9, 2008·Molecular Plant-microbe Interactions : MPMI·Yaling WangZuhua He
Feb 9, 2008·Molecular Plant-microbe Interactions : MPMI·Ajay K GoelSarah R Grant
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Christine PfundAndrew F Bent
Sep 12, 2002·Annual Review of Plant Biology·Ko Shimamoto, Junko Kyozuka
Sep 9, 2011·PLoS Pathogens·Bart W BardoelJos A G van Strijp
Sep 10, 2013·Journal of Experimental Botany·Markus Albert
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gui-In LeeSteven R Van Doren
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·A Di MatteoF Cervone
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H Shiu, A B Bleecker
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Diego WengierJorge Muschietti
Jul 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yanhai YinJoanne Chory
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Aug 28, 2012·Annual Review of Phytopathology·Meritxell Antolín-LloveraMartin Parniske

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.