Both ubiquitin ligases FBXW8 and PARK2 are sequestrated into insolubility by ATXN2 PolyQ expansions, but only FBXW8 expression is dysregulated

PloS One
Melanie Vanessa HalbachGeorg Auburger

Abstract

The involvement of the ubiquitin-proteasome system (UPS) in the course of various age-associated neurodegenerative diseases is well established. The single RING finger type E3 ubiquitin-protein ligase PARK2 is mutated in a Parkinson's disease (PD) variant and was found to interact with ATXN2, a protein where polyglutamine expansions cause Spinocerebellar ataxia type 2 (SCA2) or increase the risk for Levodopa-responsive PD and for the motor neuron disease Amyotrophic lateral sclerosis (ALS). We previously reported evidence for a transcriptional induction of the multi-subunit RING finger Skp1/Cul/F-box (SCF) type E3 ubiquitin-protein ligase complex component FBXW8 in global microarray profiling of ATXN2-expansion mouse cerebellum and demonstrated its role for ATXN2 degradation in vitro. Now, we documented co-localization in vitro and co-immunoprecipitations both in vitro and in vivo, which indicate associations of FBXW8 with ATXN2 and PARK2. Both FBXW8 and PARK2 proteins are driven into insolubility by expanded ATXN2. Whereas the FBXW8 transcript upregulation by ATXN2- expansion was confirmed also in qPCR of skin fibroblasts and blood samples of SCA2 patients, a FBXW8 expression dysregulation was not observed in ATXN2-deficient m...Continue Reading

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Citations

Feb 7, 2016·Molecular & Cellular Proteomics : MCP·David MeierhoferGeorg Auburger
Apr 28, 2018·Cellular and Molecular Neurobiology·Amin Karimi-MoghadamMohammad Reza Jabalameli
Nov 27, 2019·International Journal of Molecular Sciences·Nesli-Ece SenGeorg Auburger
May 23, 2020·The Cerebellum·Luis E Almaguer-MederosAnnelié Rodríguez-Estupiñán
Dec 27, 2016·Frontiers in Neuroscience·João M Da Conceição Alves-CruzeiroClévio Nóbrega

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Methods Mentioned

BETA
PCR
co-immunoprecipitation
transfection
Co-IP
protein assay
genotyping
biopsies
electrophoresis
fluorescence microscopy
transfections

Software Mentioned

EXCEL
AxioVision
GraphPad Prism
GraphPad
ImageJ
Zeiss

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