Both viral transcription and replication are reduced when the rabies virus nucleoprotein is not phosphorylated

Journal of Virology
Xianfu WuZhen F Fu

Abstract

Rabies virus nucleoprotein (N) plays vital roles in regulation of viral RNA transcription and replication by encapsidation of the nascent genomic RNA. Rabies virus N is phosphorylated, and previous studies demonstrated that mutation of the phosphorylated serine at position 389 to alanine resulted in reduction of viral transcription and/or replication of a rabies virus minigenome. In the present study, we mutated the serine (S) at position 389 to alanine (A), glycine (G), aspartic acid (D), asparagine (N), glutamic acid (E), and glutamine (Q) and examined the effects of these mutations on rabies virus transcription and replication in the minigenome. Furthermore, mutations from S to A, S to D, and S to E were also incorporated into the full-length infectious virus. Mutation of the serine to each of the other amino acids resulted in the synthesis of an unphosphorylated N and reduction of viral transcription and replication in the minigenome. Mutations from S to A and S to D also resulted in reduction of both viral transcription and replication in full-length infectious viruses. Growth curve studies indicated that production of the mutant virus with the S-to-A mutation (L16A) was as much as 10,000-fold less than that of the wild-ty...Continue Reading

References

Jan 24, 1991·The New England Journal of Medicine·J S SmithK Clark
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z F FuH Koprowski
Jan 1, 1990·Advances in Virus Research·A K Banerjee, D Chattopadhyay
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Mar 1, 1973·Virology·F Sokol, H F Clark
Feb 1, 1994·Journal of Virology·K K Conzelmann, M Schnell
Jan 1, 1996·Antisense & Nucleic Acid Drug Development·Z F FuH Koprowski
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·G W WertzL A Ball
May 29, 1998·The Journal of General Virology·A KouznetzoffN Tordo
Mar 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·M J SchnellR J Pomerantz

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Citations

Apr 25, 2003·Biochemical and Biophysical Research Communications·Xianfu WuZhen F Fu
Dec 1, 2009·Nature Reviews. Microbiology·Matthias J SchnellAmy Papaneri
Sep 16, 2005·Journal of Neurovirology·Jaime E CastellanosMonique Lafon
Nov 15, 2011·The Journal of Infectious Diseases·Andrea DiCarloStephan Becker
May 28, 2004·Journal of Virology·Subash C Das, Asit K Pattnaik
Feb 22, 2012·Virology Journal·Muhammad Zubair YousafSanaullah Khan
Jan 1, 2012·Uirusu·Akira Nishizono, Kentaro Yamada
Apr 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gene S TanMatthias J Schnell
Aug 8, 2007·Virus Research·Xianfu WuCharles E Rupprecht
Nov 21, 2009·Vaccine·Xianfu WuCharles E Rupprecht
Mar 31, 2015·Vaccine·Todd G SmithVictor Bronshtein
Sep 14, 2007·Virus Research·Xianfu Wu, Charles E Rupprecht
Jul 6, 2017·Vector Borne and Zoonotic Diseases·Armando de Souza PereiraElizabeth Salbé Travassos da Rosa
Oct 15, 2003·Journal of Virology·Steven A RubinKathryn M Carbone
May 10, 2019·Antiviral Research·Zoé LamaCécile Lagaudrière-Gesbert

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