Both wheat (Triticum aestivum) bran arabinoxylans and gut flora-mediated fermentation products protect human colon cells from genotoxic activities of 4-hydroxynonenal and hydrogen peroxide

Journal of Agricultural and Food Chemistry
Michael GleiBeatrice L Pool-Zobel

Abstract

Dietary fibers are fermented by the gut flora to yield short chain fatty acids (SCFAs), which inhibit the growth of tumor cells, induce glutathione S-transferases (GSTs), and protect cells from the genotoxic activity of 4-hydroxynonenal (HNE). Here, we investigated effects of wheat bran-derived arabinoxylans and fermentation products on these parameters of chemoprevention. Newly isolated water extractable (WeAx) and alkali extractable arabinoxylans (AeAx) were fermented under anaerobic conditions with human feces. Resulting fermentation supernatants (FSs) were analyzed for SCFAs and used to treat HT29 colon cancer cells. Cell growth, cytotoxicity, antigenotoxicity against hydrogen peroxide (H2O2) or HNE, and GST activity were determined. Nonfermented WeAx decreased H2O2-induced DNA damage by 64%, thus demonstrating chemoprotective properties by this nonfermented wheat bran fiber. The fermentation of WeAx and AeAx resulted in 3-fold increases of SCFA, but all FSs (including the control without arabinoxylans) inhibited the growth of the HT29 cells, reduced the genotoxicity of HNE, and enhanced the activity of GSTs (FS WeAx, 2-fold; FS AeAx, 1.7-fold; and control FS, 1.4-fold), which detoxify HNE. Thus, increases in SCFAs were not...Continue Reading

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Citations

Oct 29, 2011·European Journal of Nutrition·Wiebke SchlörmannMichael Glei
Sep 8, 2009·The British Journal of Nutrition·Anke BorowickiMichael Glei
Dec 17, 2011·The British Journal of Nutrition·Stefanie LuxMichael Glei
Feb 18, 2011·Critical Reviews in Food Science and Nutrition·Willem F BroekaertJan A Delcour
Apr 1, 2009·International Journal of Molecular Sciences·Eduardo Madrigal-SantillánEduardo Madrigal-Bujaidar
Oct 6, 2015·Biomolecules·Rudolf J SchaurPeter M Eckl
Jun 26, 2015·Toxins·Eduardo Madrigal-BujaidarEduardo Madrigal-Santillán
Nov 20, 2010·International Journal of Biological Macromolecules·Li CaoXiaobo Sun
Jul 27, 2010·New Biotechnology·Martina Newell McGloughlin
Jun 23, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Galina HovhannisyanMichael Glei
Oct 24, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S KlenowB L Pool-Zobel
Dec 7, 2007·Medicinal Research Reviews·G PoliG Leonarduzzi
May 12, 2015·TheScientificWorldJournal·Elena Franco-Robles, Mercedes G López
Oct 23, 2016·BMC Complementary and Alternative Medicine·Yuri N ClementCherrista Mohammed
Mar 5, 2019·Beneficial Microbes·V B M PetersM Meijerink
Sep 15, 2010·Free Radical Research·F GuéraudK Uchida
Sep 7, 2018·Oxidative Medicine and Cellular Longevity·Mayra Alejandra Mendez-EncinasDora Edith Valencia-Rivera
Jun 23, 2009·Journal of Agricultural and Food Chemistry·Nuria Mateo AnsonGuido R M M Haenen
Dec 7, 2007·Journal of Proteome Research·Jasmina SaricElaine Holmes

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