Bottom-up analysis using liquid chromatography-Fourier transform mass spectrometry to characterize fucosylated chondroitin sulfates from sea cucumbers

Glycobiology
Lufeng YanShiguo Chen

Abstract

Fucosylated chondroitin sulfates (FCSs) from sea cucumbers have repetitive structures that exhibit minor structural differences based on the organism from which they are recovered. A detailed characterization of FCSs and their derivatives is important to establish their structure-activity relationship in the development of new anticoagulant drugs. In the current study, online hydrophilic interaction chromatography-Fourier transform mass spectrometry (FTMS) was applied to analyze the FCS oligosaccharides generated by selective degradation from four species of sea cucumbers, Isostichopus badionotus, Pearsonothuria graeffei, Holothuria mexicana and Acaudina molpadioides. These depolymerized FCS fragments were quantified and compared using the glycomics software package, GlycReSoft. The quantified fragments mainly had trisaccharide-repeating compositions and showed significant differences in fucosylation (including its sulfation) among different species of sea cucumbers. Detailed analysis of FTMS ion peaks and top-down nuclear magnetic resonance spectroscopy of native FCS polysaccharides verified the accuracy of this method. Thus, a new structural model for FCS chains from these different sea cucumbers was defined. This bottom-up a...Continue Reading

References

Jan 1, 1989·Analytical Biochemistry·Y C Guo, H E Conrad
Sep 4, 2008·Thrombosis and Haemostasis·Bianca F GlauserPaulo A S Mourão
Sep 24, 2010·American Journal of Physiology. Renal Physiology·Nelson M Melo-FilhoPaulo A S Mourão
Aug 19, 2014·Carbohydrate Polymers·Pang MyronSujjat Al Azad
Sep 28, 2014·International Journal of Biological Macromolecules·Jie YangZhihua Lv
Jun 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Longyan ZhaoJinhua Zhao
Dec 14, 2017·European Journal of Mass Spectrometry·Isaac AgyekumI Jonathan Amster

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Methods Mentioned

BETA
FCS
nuclear magnetic resonance
NMR
FCSs

Software Mentioned

Xcalibur
GlycReSoft
Decon Tools

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