Bottom-up and top-down solid-state NMR approaches for bacterial biofilm matrix composition

Journal of Magnetic Resonance
Lynette Cegelski

Abstract

The genomics and proteomics revolutions have been enormously successful in providing crucial "parts lists" for biological systems. Yet, formidable challenges exist in generating complete descriptions of how the parts function and assemble into macromolecular complexes and whole-cell assemblies. Bacterial biofilms are complex multicellular bacterial communities protected by a slime-like extracellular matrix that confers protection to environmental stress and enhances resistance to antibiotics and host defenses. As a non-crystalline, insoluble, heterogeneous assembly, the biofilm extracellular matrix poses a challenge to compositional analysis by conventional methods. In this perspective, bottom-up and top-down solid-state NMR approaches are described for defining chemical composition in complex macrosystems. The "sum-of-the-parts" bottom-up approach was introduced to examine the amyloid-integrated biofilms formed by Escherichia coli and permitted the first determination of the composition of the intact extracellular matrix from a bacterial biofilm. An alternative top-down approach was developed to define composition in Vibrio cholerae biofilms and relied on an extensive panel of NMR measurements to tease out specific carbon pool...Continue Reading

References

Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·F H Yildiz, G K Schoolnik
May 21, 1999·Science·J W CostertonE P Greenberg
Apr 27, 2000·Journal of Bacteriology·P Watnick, R Kolter
Feb 13, 2001·Microbiology·I Sutherland
Feb 2, 2002·Science·Matthew R ChapmanScott J Hultgren
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Sep 25, 2003·International Journal of Medical Microbiology : IJMM·Ute RömlingHelmut Tschäpe
Nov 19, 2003·Biofouling·David G Allison
Mar 26, 2004·Nature Reviews. Microbiology·Luanne Hall-StoodleyPaul Stoodley
Nov 17, 2009·Nature Chemical Biology·Lynette CegelskiScott J Hultgren
Mar 17, 2010·Applied and Environmental Microbiology·Yongqin JiaoMichael P Thelen
Aug 3, 2010·Nature Reviews. Microbiology·Hans-Curt Flemming, Jost Wingender
May 4, 2011·Applied Microbiology and Biotechnology·Esther KarunakaranCatherine A Biggs
Aug 9, 2011·Nature Reviews. Microbiology·Jeramie D Watrous, Pieter C Dorrestein
Jul 18, 2012·Future Medicinal Chemistry·Bo Zhang, Robert Powers
Dec 25, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Cynthia WuLynette Cegelski
Jul 6, 2013·Journal of Molecular Biology·Oscar A McCrateLynette Cegelski
Nov 19, 2013·Biochemical and Biophysical Research Communications·Ji Youn LimLynette Cegelski
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Emrah AltindisJohn J Mekalanos
Jun 10, 2014·Biochimica Et Biophysica Acta·Courtney ReichhardtLynette Cegelski
Jul 1, 2014·Molecular Physics·Courtney Reichhardt, Lynette Cegelski
Jul 30, 2014·Current Opinion in Microbiology·Jinshu Fang, Pieter C Dorrestein
Nov 25, 2014·Biophysical Journal·Emily C HollenbeckLynette Cegelski

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Citations

Sep 16, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Joseph A H Romaniuk, Lynette Cegelski
Jun 17, 2015·Trends in Microbiology·Ute Römling, Michael Y Galperin
Aug 4, 2016·Future Medicinal Chemistry·Courtney ReichhardtLynette Cegelski
Mar 16, 2016·Journal of Bacteriology·Karen L VisickJean-Marc Ghigo
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Aug 4, 2019·Solid State Nuclear Magnetic Resonance·Noelle I RabiahLynette Cegelski
Apr 5, 2017·Dental Materials : Official Publication of the Academy of Dental Materials·Nancy J Lin
Oct 27, 2017·Biochemistry·Christopher BartlettJoel T Weadge

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