Botulinum neurotoxin type A modulates vesicular release of glutamate from satellite glial cells

Journal of Cellular and Molecular Medicine
Larissa Bittencourt da SilvaParisa Gazerani

Abstract

This study investigated the presence of cell membrane docking proteins synaptosomal-associated protein, 25 and 23 kD (SNAP-25 and SNAP-23) in satellite glial cells (SGCs) of rat trigeminal ganglion; whether cultured SGCs would release glutamate in a time- and calcium-dependent manner following calcium-ionophore ionomycin stimulation; and if botulinum neurotoxin type A (BoNTA), in a dose-dependent manner, could block or decrease vesicular release of glutamate. SGCs were isolated from the trigeminal ganglia (TG) of adult Wistar rats and cultured for 7 days. The presence of SNAPs in TG sections and isolated SGCs were investigated using immunohistochemistry and immunocytochemistry, respectively. SGCs were stimulated with ionomycin (5 μM for 4, 8, 12 and 30 min.) to release glutamate. SGCs were then pre-incubated with BoNTA (24 hrs with 0.1, 1, 10 and 100 pM) to investigate if BoNTA could potentially block ionomycin-stimulated glutamate release. Glutamate concentrations were measured by ELISA. SNAP-25 and SNAP-23 were present in SGCs in TG sections and in cultured SGCs. Ionomycin significantly increased glutamate release from cultured SGCs 30 min. following the treatment (P < 0.001). BoNTA (100 pM) significantly decreased glutamate ...Continue Reading

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Citations

Mar 30, 2018·Toxins·Parisa Gazerani
Apr 5, 2019·Current Treatment Options in Neurology·Marc E Lenaerts, Tiffany H Green
Feb 7, 2018·Acta Neurologica Scandinavica·T P DoH W Schytz
May 23, 2018·Toxins·Elena FonfriaJohannes Krupp
Aug 31, 2018·Neuronal Signaling·Raj Kumar
Aug 8, 2019·Toxins·Ivica MatakZsuzsanna Helyes
May 18, 2018·Frontiers in Neurology·Nádia Pereira GonçalvesLone Tjener Pallesen
Jun 22, 2021·Cephalalgia : an International Journal of Headache·Tarique Benbow, Brian E Cairns
Jul 25, 2021·International Journal of Molecular Sciences·Juliette Duchesne de LamotteCamille Nicoleau

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Methods Mentioned

BETA
Fluorescence microscopy

Software Mentioned

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