PMID: 3753681Jan 15, 1986Paper

Botulinum neurotoxin type B. Its purification, radioiodination and interaction with rat-brain synaptosomal membranes

European Journal of Biochemistry
D M EvansJ O Dolly

Abstract

Neurotoxin from Clostridium botulinum type B was purified to homogeneity by by affinity and ion-exchange chromatography; specific neurotoxicity of this protein (Mr of approximately equal to 155 000) following trypsinisation attained a level of 2 X 10(8) mouse LD50 units/mg protein. 125I-iodination of the toxin to high specific radioactivities (19-63 TBq/mmol) yielded typically greater than 65% of its original toxicity; dodecyl sulphate gel electrophoresis under reducing conditions, after trypsinisation, showed that the larger polypeptide (Mr of approximately equal to 101 000) was labelled preferentially. Saturable binding of the 125I-labelled neurotoxin to rat cerebrocortical synaptosomes was observed and Scatchard analysis showed a low content of acceptors with high affinity (Kd = 0.3-0.5 nM;Bmax approximately equal to 30-60 fmol/mg protein, together with a much larger population of weak-affinity sites. No significant differences in binding affinity were seen in competition experiments using native or fully activated (trypsinized) neurotoxin, indicating that chain cleavage is not essential for acceptor-toxin interaction. Type A botulinum neurotoxin showed a limited capacity to inhibit the synaptosomal binding of labelled type ...Continue Reading

References

Nov 1, 1976·Naunyn-Schmiedeberg's Archives of Pharmacology·M Kitamura
Jul 31, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·S Kozaki
May 1, 1978·Applied and Environmental Microbiology·L J Moberg, H Sugiyama
Oct 1, 1978·Journal of Neurochemistry·C B Gundersen, B D Howard
May 1, 1975·Infection and Immunity·S Kozaki, G Sakaguchi
Sep 1, 1976·Infection and Immunity·B R Dasgupta, H Sugiyama
Jan 1, 1989·Journal of Neural Transmission. Parkinson's Disease and Dementia Section·S LandeD W-Wray
Jan 1, 1985·Toxicon : Official Journal of the International Society on Toxinology·A C AshtonJ O Dolly
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·B Weeke
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, M A Woody
Feb 1, 1980·European Journal of Biochemistry·M MonsignyF Delmotte
Nov 23, 2006·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·J O Dolly, K R Aoki

❮ Previous
Next ❯

Citations

Oct 1, 2003·Current Treatment Options in Neurology·Charles E. Argoff
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·A C AshtonJ O Dolly
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·N YokosawaK Oguma
Dec 1, 1992·Brain Research Bulletin·C L SchengrundB R Dasgupta
Mar 24, 2004·Toxicon : Official Journal of the International Society on Toxinology·Hai-Yuan ChaoHwan-Wun Liu
Dec 24, 1997·Toxicon : Official Journal of the International Society on Toxinology·L B PearceA Gupta
Jan 5, 2002·Journal of Physiology, Paris·Frédéric A MeunierJordi Molgó
Jul 4, 2012·Journal of Biomolecular Structure & Dynamics·Sureshkumar Venkateshwari, Kasinadar Veluraja
Feb 7, 2003·The Clinical Journal of Pain·Paulette E Setler
Jun 15, 1987·European Journal of Biochemistry·J Sanchez-PrietoD G Nicholls
Mar 18, 2010·Annual Review of Biochemistry·Mauricio Montal
Dec 3, 2010·PLoS Pathogens·Felix L YehEdwin R Chapman
Sep 1, 1991·Journal of Neurochemistry·C L SchengrundN J Ringler
Nov 1, 1995·Quarterly Reviews of Biophysics·C Montecucco, G Schiavo
May 20, 2011·The FEBS Journal·Subramanyam Swaminathan
Jan 15, 2004·Anesthesiology Clinics of North America·P Prithvi Raj
Apr 6, 2004·Facial Plastic Surgery Clinics of North America·Samuel M Lam
Mar 18, 2004·Movement Disorders : Official Journal of the Movement Disorder Society·Subramanyam SwaminathanDesigan Kumaran
Sep 25, 2003·The Journal of Cell Biology·Min DongEdwin R Chapman
Jun 21, 2006·Neurotoxicity Research·K A FosterK R Aoki
Apr 4, 2000·Physiological Reviews·G SchiavoC Montecucco
Jun 3, 2018·Toxins·Jihye Park, Myung Eun Chung
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryoki KobayashiKohtaro Fujihashi
Jun 1, 1997·Infection and Immunity·N M BakryL L Simpson
Mar 1, 1992·Microbiological Reviews·E J Schantz, E A Johnson
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Judit Herreros, Giampietro Schiavo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.