Bovine activin receptor type IIB messenger ribonucleic acid displays alternative splicing involving a sequence homologous to Src-homology 3 domain binding sites

Endocrinology
J F EthierD W Silversides

Abstract

Activins are implicated in a variety of biological effects, particularly in reproductive processes such as embryonic development and folliculogenesis. Breakthroughs in the elucidation of the activin signal transduction mechanism were achieved with the characterization of the activin receptors, and the recent identification of cytoplasmic factors apparently involved in the signaling process. The present studies were undertaken to further analyze the activin signaling pathway. The complementary DNA coding for the bovine activin receptor type IIB (bActRIIB) was amplified by RT-PCR from corpus luteum and pituitary RNA, and cloned to characterize its role in activin signal transduction. Two complementary DNA isoforms (bActRIIB2 and bActRIIB5) were detected, coding for 512 amino acids and 498 amino acids, respectively. The shortest isoform lacked a sequence encoding a 14-amino acid stretch very rich in proline residues, located between the transmembrane region and the intracellular kinase domain. Intron sequencing and ribonuclease protection assay demonstrated that alternative splicing is responsible for the generation of these bActRIIB isoforms. This alternative splicing event is unique in that it has not been observed in other spec...Continue Reading

Citations

Dec 6, 2008·General and Comparative Endocrinology·Yamila CarpioMario Pablo Estrada
Jun 1, 2007·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Tone-Kari K OstbyeOivind Andersen
Feb 28, 2003·Biology of Reproduction·Joëlle DupontCatherine Taragnat
Aug 19, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C Peng, S T Mukai
Feb 22, 2000·The Journal of Biological Chemistry·H ShojiH Sugino

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