Bovine adrenals contain, in addition to ouabain, a second inhibitor of the sodium pump.

The Journal of Biological Chemistry
R SchneiderW Schoner

Abstract

In the search for endogenous cardiac glycosides, two different inhibitors of the sodium pump have been isolated from bovine adrenals. Inhibitor A with a molecular mass of 600 Da and a UV maximum at 250 nm was purified from 16 kg of bovine adrenals. The pure substance (<1 ng) inhibited the sodium pump of human red blood cells with an affinity similar to that of ouabain, yet it cross-reacted with antibodies against the bufadienolide proscillaridin A but not against the cardenolide ouabain. Inhibitor A was slightly more hydrophilic than ouabain on RP-C18 high pressure liquid chromatography. Hence, it showed properties similar to the proscillaridin A immunoreactivity (Sich, B., Kirch, U., Tepel, M., Zideck, W., and Schoner, W. (1996) Hypertension 27, 1073-1078) that increased in humans with systolic blood pressure and pulse pressure. Inhibitor B of the sodium pump with a molecular mass of 584 Da was purified 10(6)-fold from 20 kg of bovine adrenals. It cross-reacted with antibodies against ouabain but not with antibodies against proscillaridin A and inhibited the sodium pump of human and rat red blood cells with the same affinity as ouabain. All other properties, such as the retention time in a C18-reversed phase chromatography, mo...Continue Reading

References

Jun 1, 1992·Hypertension·J H LudensD W DuCharme
Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M HamlynJ H Ludens
Dec 31, 1990·Biochemical and Biophysical Research Communications·A GotoT Sugimoto
Jul 1, 1985·Physiological Reviews·H E de Wardener, E M Clarkson
Aug 1, 1994·Endocrinology·J LaredoJ M Hamlyn
Jan 15, 1993·European Journal of Biochemistry·M TamuraT Inagami
Aug 15, 1993·European Journal of Biochemistry·D LichtsteinJ Deutsch
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A A TymiakE Haber
Aug 3, 1996·Lancet·P J HiltonW E Morden
Jan 3, 1997·Molecular and Cellular Endocrinology·G A LeBlancV S Wilson
Jan 3, 1997·Molecular and Cellular Endocrinology·A PerrinG Defaye

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Citations

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Dec 4, 2008·The Journal of Membrane Biology·P F OliveiraA Rebelo da Costa
Jun 11, 2008·Nature Clinical Practice. Nephrology·Alexei Y Bagrov, Joseph I Shapiro
Oct 25, 2008·Nature Reviews. Drug Discovery·Ioannis Prassas, Eleftherios P Diamandis
May 25, 2002·European Journal of Biochemistry·Wilhelm Schoner
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Luc DesfrerePer Uhlén
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Isabel LarreMarcelino Cereijido
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Isabel LarreMarcelino Cereijido
Jun 17, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Wilhelm Schoner, Georgios Scheiner-Bobis
Feb 24, 2001·Journal of Hypertension·Y KomiyamaH Takahashi
May 23, 2002·Journal of Hypertension·Daniele Cusi
Apr 9, 2004·Journal of Cardiovascular Pharmacology·Giuseppina D'UrsoUmberto Montali
Apr 11, 2000·Current Opinion in Nephrology and Hypertension·M Ferrandi, P Manunta
Dec 4, 2008·Journal of Hypertension·Paolo ManuntaJohn M Hamlyn
Aug 18, 2009·Journal of Hypertension·Tatiana KuznetsovaJan A Staessen
Mar 11, 2004·European Journal of Biochemistry·Robert Saunders, Georgios Scheiner-Bobis
Mar 16, 2011·Clinical and Experimental Pharmacology & Physiology·Masamichi YoshikaHakuo Takahashi
Mar 28, 2009·Pharmacological Reviews·Alexei Y BagrovOlga V Fedorova
Nov 8, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Mordecai P BlausteinW Gil Wier
Dec 22, 2004·Pediatric Research·Philip A GruppusoTheresa C Bienieki
Jul 6, 2007·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Wilhelm Schoner, Georgios Scheiner-Bobis
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A KawamuraK Nakanishi
Sep 3, 2011·The Journal of Microbiology·Fatih Saban BerisAli Osman Belduz
Dec 18, 2013·The Journal of Physiology·Hong SongMordecai P Blaustein
Feb 8, 2005·The Journal of Physiology·Hai LinMasumi Inoue
Apr 28, 2006·Kidney International·T Iwamoto, S Kita
Apr 13, 2010·Toxicon : Official Journal of the International Society on Toxinology·Geraldino A Cunha-FilhoMaria L Dos Santos
Jul 15, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mordecai P Blaustein
May 24, 2003·Annals of the New York Academy of Sciences·John M HamlynBruce P Hamilton
May 24, 2003·Annals of the New York Academy of Sciences·Wilhelm SchonerErnst-Güngther Grünbaum
Jul 22, 2006·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Olga AkimovaSergei N Orlov
Jul 11, 2006·Life Sciences·Francis J Haddy
Sep 6, 2005·Seminars in Nephrology·Wilhelm Schoner, Georgios Scheiner-Bobis
Mar 30, 2010·Biochimica Et Biophysica Acta·Olga V FedorovaAlexei Y Bagrov
Mar 17, 2010·Biochimica Et Biophysica Acta·Paolo ManuntaJohn M Hamlyn
Mar 17, 2010·Biochimica Et Biophysica Acta·Frans H H Leenen

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