PMID: 8611630Apr 10, 1996Paper

Bovine alpha 1-microglobulin/bikunin. Isolation and characterization of liver cDNA and urinary alpha 1-microglobulin

Biochimica Et Biophysica Acta
A Lindqvist, B Akerström

Abstract

cDNA coding for alpha 1-microglobulin, an immunoregulatory plasmaprotein, was isolated from bovine liver. The sequence of a total of 1258 nucleotides revealed an open reading frame of 352 amino acids. This included alpha 1-microglobulin, 182 amino acids, and bikunin, the light chain of the plasmaprotein inter-alpha-inhibitor, 147 amino acids. The two proteins were connected by a basic tetrapeptide, R-A-R-R, which conforms to the consensus sequence recognized by endoproteolytic cleavage enzymes. The deduced amino acid sequence showed a high degree of identity with alpha 1-microglobulin and bikunin sequences from other species, and the alpha 1-microglobulin part displayed sequence motifs typical for members of the lipocalin protein superfamily. A single alpha 1-microglobulin/bikunin mRNA with a size of around 1300 nt was found in bovine liver. The mature alpha 1-microglobulin protein was isolated from bovine urine, and partly characterized. It was found to be a globular molecule with an apparent molecular weight of 23,300, containing one N-linked and at least on O-linked oligosaccharide, one intra-chain disulfide bridge and an electrophoretic heterogeniety with a pI-value of 4.1-5.2.

References

Jun 1, 1990·Trends in Biochemical Sciences·B Akerström, L Lögdberg
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, U Lindahl
Dec 30, 1987·European Journal of Biochemistry·B AkerströmL Rask
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M A BourdonE Ruoslahti
Aug 1, 1994·The Biochemical Journal·C FalkenbergB Akerström
Mar 31, 1994·Biochimica Et Biophysica Acta·J K PerryC A Tse
Aug 1, 1995·Protein Expression and Purification·T Bratt, B Akerström
Apr 1, 1994·Physical Review. B, Condensed Matter·P MendelsC E Stronach

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Citations

May 18, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K YoshidaH Sinohara
Nov 16, 2004·Archives of Biochemistry and Biophysics·Jörgen LarssonBo Kerström
Jan 13, 1999·The Japanese Journal of Thoracic and Cardiovascular Surgery : Official Publication of the Japanese Association for Thoracic Surgery = Nihon Kyōbu Geka Gakkai Zasshi·T NarisawaT Takaba
Oct 4, 2020·International Journal of Molecular Sciences·Amanda KristianssonJill R Storry
Mar 23, 2021·Frontiers in Physiology·Jesper BergwikBo Åkerström
Nov 4, 2000·Biochimica Et Biophysica Acta·B AkerströmA Lindqvist

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