Abstract
The genomic architecture and expression of the Igf-1 gene are complex yielding multiple IGF-I transcript isoforms with putative functional contributions to growth and metabolism. Using RNA-seq on different tissues, physiological states, and species, the breadth of transcripts expressed was determined. Tissues from pre- and post-pubertal heifers and mature mice were collected and the transcript isoforms characterized. Three different IGF-I isoforms were detected in heifers with Class 1 transcripts most abundantly expressed. The pituitary reduced IGF-I expression post-pubertally whereas the uterus increased expression. Murine IGF-I transcript expression was more diverse utilizing multiple exons, start sites, and 3'UTRs. The RNA-seq methodology to characterize expression profiles permits assessment of the transcript isoforms yielding insight into functional roles of each transcript.
References
Dec 11, 1992·Endocrinology·J ShemerD LeRoith
Nov 11, 1991·Molecular Endocrinology·M L AdamoD LeRoith
Apr 30, 1991·Biochemical and Biophysical Research Communications·M L AdamoC T Roberts
Mar 1, 1987·Molecular Endocrinology·C T RobertsD LeRoith
Jan 1, 1994·The Journal of Endocrinology·R S Gilmour
Aug 4, 1999·Molecular and Cellular Endocrinology·L ShobaW L Lowe
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·W W Lin, A M Oberbauer
Dec 3, 2002·Gene·Marisa L WongJuan F Medrano
Dec 4, 2003·Domestic Animal Endocrinology·Y WangH Jiang
Feb 11, 2005·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Thea ShavlakadzeMiranda D Grounds
Jun 7, 2007·Zoological Science·Takashi OhtsukiSumio Takahashi
Jun 22, 2007·Neurochemistry International·Dariusz C GóreckiBarbara Zabłocka
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Sep 22, 2009·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Małgorzata BeresewiczDariusz C Górecki
Feb 16, 2010·Journal of Animal Science·P Luna-NevarezM G Thomas
Aug 6, 2010·Endocrinology·Masanobu KawaiClifford J Rosen
Nov 9, 2010·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Angela CánovasJuan F Medrano
Jan 27, 2012·BMC Genomics·Saumya WickramasingheJuan F Medrano
Mar 15, 2012·Journal of Neurotrauma·Michelle E SchoberRobert H Lane
Mar 28, 2013·Frontiers in Endocrinology·A M Oberbauer
Apr 2, 2013·Frontiers in Endocrinology·Becky K Brisson, Elisabeth R Barton
Citations
Oct 1, 2014·Nature Communications·Armin RehmUta E Höpken
Mar 17, 2015·Journal of Animal Science and Biotechnology·Anita M Oberbauer
Apr 7, 2016·Biochimica Et Biophysica Acta·Giosuè AnnibaliniElena Barbieri
Jun 19, 2019·Animal Reproduction Science·Trish BergerHeidi Kucera