Bovine bone particulates containing bone anabolic factors as a potential xenogenic bone graft substitute

Journal of Orthopaedic Surgery and Research
David S MussonJillian Cornish

Abstract

Alternative grafts are needed to improve the healing of bone non-union. Here, we assessed a bovine bone product which retains the inorganic and organic components of bone, as an alternative bone graft. Bovine bone matrix proteins (BBMPs) were isolated from bovine bone particulates (BBPs) and tested in vitro. Primary rat osteoblast viability, differentiation, and mineralisation were assessed with alamarBlue®, real-time PCR, and von Kossa staining assays, respectively. Osteoclast formation was assessed in primary murine bone marrow cultures with TRAP staining. Human osteoblast growth and differentiation in the presence of BBPs was evaluated in 3D collagen gels in vitro using alamarBlue® and real-time PCR, respectively. The efficacy of BBPs as an alternative bone graft was tested in a rat critical-size calvarial defect model, with histology scored at 4 and 12 weeks post-surgery. In vitro, the highest concentration of BBMPs increased mineral deposition five-fold compared to the untreated control group (P < 0.05); enhanced the expression of key osteoblast genes encoding for RUNX2, alkaline phosphatase, and osteocalcin (P < 0.05); and decreased osteoclast formation three-fold, compared to the untreated control group (P < 0.05). Howev...Continue Reading

References

Jan 1, 1991·Life Sciences·J J StepanD J Baylink
Dec 1, 1988·Calcified Tissue International·E CanalisM Centrella
Aug 1, 1988·The Journal of Clinical Investigation·J PfeilschifterG R Mundy
Nov 1, 1971·Journal of Dental Research·M R Urist, B S Strates
Nov 12, 1965·Science·M R Urist
Jan 23, 1999·International Orthopaedics·E Solheim
Dec 22, 1999·Pain·Nikolaos Maniadakis, Alastair Gray
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 3, 2007·The Journal of Bone and Joint Surgery. American Volume·William G De LongTracy Watson
Apr 1, 2008·The Spine Journal : Official Journal of the North American Spine Society·Wellington K Hsu, Jeffrey C Wang
Aug 30, 2008·HSS Journal : the Musculoskeletal Journal of Hospital for Special Surgery·Mathias P G Bostrom, Daniel A Seigerman
Jan 15, 2010·Clinical Oral Implants Research·Mauricio G AraújoJan Lindhe
Oct 13, 2010·Annual Review of Pathology·Xu Feng, Jay M McDonald
Oct 21, 2010·Cellular Signalling·Beth BragdonAnja Nohe
Feb 4, 2011·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·A S BrydoneS Maclaine
Jul 7, 2011·The Spine Journal : Official Journal of the North American Spine Society·Eugene J CarrageeBradley K Weiner
Oct 27, 2012·The Spine Journal : Official Journal of the North American Spine Society·Garet C ComerEugene J Carragee
Feb 5, 2013·The International Journal of Oral & Maxillofacial Implants·Laura M E ScheerlinckEllen M Van Cann
Jan 18, 2014·Craniomaxillofacial Trauma & Reconstruction·Manar AlmaimanPaul Manson
Aug 15, 2014·BoneKEy Reports·Brya G MatthewsJillian Cornish
Jun 22, 2016·Clinical Implant Dentistry and Related Research·Jung-Seok LeeKyoo-Sung Cho
May 1, 2018·The International Journal of Artificial Organs·Zeljka Perić KačarevićMike Barbeck

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
chips
PCR

Software Mentioned

BIOQUANT OSTEO
BIOQUANT Image

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao
L Chen, N T Foged
Biochemical and Biophysical Research Communications
Hannu-Ville LeskeläPetri Lehenkari
© 2022 Meta ULC. All rights reserved