Abstract
With the aid of a horseradish peroxidase (HRP) tagged monoclonal antibody against smooth lipopolysaccharide from Brucella abortus (Bruce 1), a competitive and superimposable ELISA test procedure for bovine brucellosis has been evaluated for its ability to discriminate between Strain 19-vaccinated (S19-Vacc) and Biotype 1-infected (B1-Inf) cattle. In the competitive assay, all sera from S19-Vacc animals competed effectively against HRP-Bruce 1 (low HRP activity), while 10 out of 40 B1-Inf animals competed less effectively with Bruce 1 (high HRP activity). Successful competition by cattle antibodies would result in an increased proportion of cattle Igs binding to the assay antigen. This was confirmed by superimposing an alkaline phosphatase conjugated rabbit anti-cattle Ig after the competitive ELISA had been completed. With the superimposable assay, alkaline phosphatase activity was correspondingly high for S19-Vacc animals, and low for 36 out of 40 B1-Inf animals. The superimposable ELISA had therefore improved the discriminatory capabilities of the assay procedure from 75% to 90%.
References
Mar 1, 1976·Australian Veterinary Journal·P PlackettS J Best
Feb 1, 1985·Veterinary Immunology and Immunopathology·P G BundesenD B Rylatt
Feb 1, 1987·Australian Veterinary Journal·R Crichton, N E Medveczky
Feb 1, 1985·Veterinary Immunology and Immunopathology·D B RylattP G Bundesen
Sep 15, 1973·The Veterinary Record·R Diaz, L M Jones
Jun 1, 1974·The Veterinary Record·W J Morgan, R A Richards
Nov 1, 1984·Molecular Immunology·W L Cleveland, B F Erlanger
Apr 1, 1982·The Biochemical Journal·J J Chin
Oct 1, 1980·Scientific American·C Milstein
Jan 1, 1981·Methods in Enzymology·M J O'Sullivan, V Marks
Dec 1, 1984·Veterinary Microbiology·S S Sutherland
Sep 1, 1983·Australian Veterinary Journal·J C Chin
Oct 1, 1981·Journal of Clinical Microbiology·D K BorakerJ R Kunkel
Feb 1, 1970·Infection and Immunity·D LeongJ B Wilson
Citations
Sep 6, 2008·Veterinary Immunology and Immunopathology·K NielsenT Renteria
May 1, 2007·Veterinary Microbiology·K NielsenT Renteria
Feb 2, 2006·Analytical Chemistry·Joung-Hwan ChoSe-Hwan Paek