Bovine caveolin-2 cloning and effects of shear stress on its localization in bovine aortic endothelial cells

Endothelium : Journal of Endothelial Cell Research
Nolan L BoydH Jo

Abstract

Caveolae are plasmalemmal domains enriched with cholesterol, caveolins, and signaling molecules. Normally, cells that express caveolin-1 also express caveolin-2, but this has not been demonstrated in bovine aortic endothelial cells (BAECs). Here, we show that BAECs express caveolin-2, which localizes in caveolae with caveolin-1. We have cloned the bovine caveolin-2 gene and after comparison with known protein sequences (human, murine, rat, and canine) have found divergent immunogenic regions (amino acid [aa] 21 to aa 50 and aa 79 to 88), which may explain the inability to detect caveolin-2 in different cell types. We developed a bovine caveolin-2-specific antibody to examine this protein's expression and localization in BAECs. We used differential gradient centrifugations and immunoprecipitation to show that bovine caveolin-2 and caveolin-1 form a hetero-oligomer in plasma membrane caveolae. Using immunocytochemistry we show that a pool of caveolin-2 also colocalizes with the cis-Golgi in static culture, but unlike caveolin-1, this Golgi associated pool is maintained after 1 day of shear exposure. Therefore, the interaction of caveolin-2 with caveolin-1 could play an important role in caveolae biogenesis and shear stimulated me...Continue Reading

References

Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J SmartR G Anderson
Sep 1, 1993·Journal of Molecular Graphics·J L Pellequer, E Westhof
Jul 1, 1995·Physiological Reviews·P F Davies
Mar 21, 1998·The Journal of Cell Biology·P ScheiffeleE Ikonen
Oct 3, 1998·The Journal of Biological Chemistry·V RizzoJ E Schnitzer
Apr 6, 2000·American Journal of Physiology. Heart and Circulatory Physiology·H ParkH Jo
Dec 21, 2000·American Journal of Physiology. Heart and Circulatory Physiology·N AzumaB E Sumpio
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Garcia-CardeñaM A Gimbrone
Mar 2, 2002·Immunology·James HarrisChris J Howard
Mar 9, 2002·Molecular and Cellular Biology·Babak RazaniMichael P Lisanti
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·You-Yang ZhaoKenneth R Chien
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Grzegorz SowaWilliam C Sessa
May 24, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Nolan L BoydHanjoong Jo
Jun 21, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Victor RizzoPeter F Davies

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.

© 2021 Meta ULC. All rights reserved