Bovine colostrum promotes growth and migration of the human keratinocyte HaCaT cell line

Growth Factors
Daniela KovacsMauro Picardo

Abstract

Bovine colostrum represents a rich source of growth factors, which are known to play a central role in wound healing. The aim of our study was to investigate the possible mitogenic and motogenic effects induced by colostrum on human keratinocytes. Cell proliferation evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide test and 5-Bromo-2'-deoxyuridine incorporation revealed that colostrum exerts a growth promoting activity. Scratch assay and immunofluorescence of actin cytoskeleton showed its effectiveness also in inducing cell migration. Furthermore, colostrum treatment increases the levels of tyrosine phosphorylated proteins and the activated forms of the extracellular signal-regulated kinases 1 and 2 and such effects appear to be repressed by the tyrosine kinase inhibitor genistein. Our results indicate that the biological activities of colostrum are specifically mediated by the growth factor-induced activation of tyrosine kinase receptors and underline the relevance of the synergistic action exerted by the growth factors in stimulating keratinocyte proliferation and migration essential for tissue repair.

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Citations

Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Federico MussanoBenedetta Bussolati
Jul 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Oscar SassoDaniele Piomelli
Mar 12, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniela KovacsGiorgia Cardinali

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