PMID: 6981877Jul 1, 1982Paper

Bovine costimulator. I. Production kinetics, partial purification, and quantification in serum-free Iscove's medium

Veterinary Immunology and Immunopathology
P E Baker, K F Knoblock

Abstract

Bovine peripheral blood leukocytes were examined for blast transformation in response to T-cell lectins in serum-containing RPMI 1640 medium and serum-free Iscove's medium. Phytohemagglutinin-induced blastogenesis was significantly greater in Iscove's medium than in RPMI containing ten percent fetal calf serum. Concanavalin A-induced blast transformation was equivalent in both media. However, the kinetics of lectin response and the quantity of lectin required for optimum blastogenesis was considerably different in the two culture media. Concanavalin A-induced blast transformation of bovine thymocytes in Iscove's medium revealed that a concentration of 10(6) cells/ml, inconsequential blastogenesis ensued; but at 10(7) cells/ml blast transformation was significant and dose-dependent. Therefore, conditioned media from concanavalin A-stimulated bovine peripheral blood leukocytes, prepared in serum-free Iscove's medium, were assayed for costimulator activity using bovine thymocytes at 10(6) cells/ml in Iscove's medium as indicator cells. Both optimum lectin requirements and cell concentrations for production of costimulator activity were found. Conditioned medium, operated with the total exclusion of serum and with optimal costimula...Continue Reading

References

Jan 1, 1977·Immunological Reviews·F H BachM L Bach
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Citations

Jan 1, 1988·Comparative Immunology, Microbiology and Infectious Diseases·B W FenwickB I Osburn
Jul 1, 1982·Veterinary Immunology and Immunopathology·P E Baker, K F Knoblock
Sep 1, 1984·Veterinary Immunology and Immunopathology·M Miller-Edge, G A Splitter
Jan 1, 1984·Veterinary Immunology and Immunopathology·L C GasbarreR D Romanowski
Jul 1, 1984·Veterinary Immunology and Immunopathology·S Cerruti-SolaA L de Weck
Oct 1, 1984·Veterinary Immunology and Immunopathology·G Oldham, L Williams
Jan 1, 1985·Veterinary Immunology and Immunopathology·J A Ellis, J C DeMartini
Dec 1, 1986·Veterinary Immunology and Immunopathology·H Bielefeldt Ohmann, L A Babiuk
Sep 1, 1987·Veterinary Immunology and Immunopathology·K A Knisley, L D Pearson
Oct 1, 1988·Veterinary Immunology and Immunopathology·R M Bauer, R G Olsen
Jun 1, 1988·Veterinary Immunology and Immunopathology·P T Zelarney, E L Belden
Dec 1, 1989·Veterinary Immunology and Immunopathology·B ArunachalamG C Ram
Mar 27, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Dhanjit Kumar DasPraveen Kumar Gupta
Jan 1, 1986·Infection and Immunity·H Bielefeldt Ohmann, L A Babiuk

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