Bovine Decellularized Amniotic Membrane: Extracellular Matrix as Scaffold for Mammalian Skin

Polymers
Andrea Catalina Villamil BallesterosYuli Alexandra Neira Bejarano

Abstract

Decellularized membranes (DM) were obtained from bovine amniotic membranes (BAM) using four different decellularization protocols, based on physical, chemical, and mechanical treatment. The new material was used as a biological scaffold for in vitro skin cell culture. The DM were characterized using hematoxylin-eosin assay, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR-ATR), and differential scanning calorimetry (DSC). The in vitro cytotoxicity of DM was evaluated using MTT. The efficacy of decellularization process was assessed through DNA quantification and electrophoresis. All the used protocols showed a high effectiveness in terms of elimination of native cells, confirmed by DNA extraction and quantification, electrophoresis, and SEM, although protocol IV removes the cellular contents and preserve the native extracellular matrix (ECM) architecture which it can be considered as the most effective in terms of decellularization. FTIR-ATR and DSC on the other hand, revealed the effects of decellularization on the biochemical composition of the matrices. There was no cytotoxicity and the biological matrices obtained were a source of collagen for recellularization. The matrices of protocols I, ...Continue Reading

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Citations

Apr 8, 2020·Polymers·Marián Lehocký, Petr Humpolíček
Jul 13, 2021·Advanced Materials·Mahshid KharazihaNasim Annabi
Sep 1, 2021·Journal of Tissue Engineering and Regenerative Medicine·Eren OzudogruBurak Derkus

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Methods Mentioned

BETA
xenografts
electrophoresis
PCR
biopsy
Assay
differential scanning calorimetry

Software Mentioned

Mettler
Toledo Stare
OMNIC

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