PMID: 590970Nov 1, 1977

Bovine fetal fluid cells in vitro: fate and fetal sex prediction accuracy

In Vitro
A Bongso, P K BASRUR

Abstract

The in vitro fate of bovine fetal fluid cells and the efficiency of fetal sex predication from cultures of these cells are studied using aspirates from live animals and pregnant uteri collected from the slaughterhouse. Over 70 percent of bovine amniotic fluid samples aspirated from pregnant uteri at the time of slaughter yielded cultures adequate for chromosome analysis whereas only 10 percent of allantoic fluid samples produced growth of cells satisfactory for chromosome analysis. Fetal sexing accuracy was 100 percent in all samples studied. Seven readily recognizable cell types were noted in cultures of fetal fluid cells obtained at various stages of gestation. In a majority of cases, the in vitro morphology of cells from both fetal cavities was similar to that observed in primary human amniotic fluid cell cultures.

References

Mar 1, 1976·Journal of Reproduction and Fertility·T A BongsoE V YoungLai
Jun 1, 1975·Clinical Obstetrics and Gynecology·R H Schwarz
Feb 8, 1975·The Veterinary Record·T A Bongso, P K BASRUR
Nov 10, 1973·Lancet·G R SutherlandJ B Scrimgeour
Jul 15, 1973·American Journal of Obstetrics and Gynecology·L L CederqvistD J Rothe
Sep 1, 1973·Journal of Clinical Pathology·R G ChithamL Hill
Jun 1, 1974·Journal of Medical Genetics·G R SutherlandA D Bain
Jul 1, 1971·The Journal of Pediatrics·C GrayM M Cohen
Feb 28, 1970·British Medical Journal·M M Nelson, A E Emery
Feb 15, 1968·American Journal of Obstetrics and Gynecology·R A PattilloR F Mattingly
Feb 1, 1968·Obstetrics and Gynecology·A B GerbieJ I Brewer
Nov 1, 1960·Experimental Cell Research·I R KONIGSBERG
Dec 26, 1964·Nature·P K BASRUR, J P GILMAN

Related Concepts

Structure of Allantois
Amniotic Fluid Index
Metazoa
Bos indicus
Cell Density
Cell Division Phases
Chromosomes
Epithelial Cells
Extraembryonic Membranes
Fibroblasts

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