PMID: 9525625Apr 3, 1998Paper

Bovine herpesvirus 1 glycoprotein M forms a disulfide-linked heterodimer with the U(L)49.5 protein

Journal of Virology
S X WuG J Letchworth

Abstract

Nine glycoproteins (gB, gC, gD, gE, gG, gH, gI, gK, and gL) have been identified in bovine herpesvirus 1 (BHV-1). gM has been identified in many other alpha-, beta-, and gammaherpesviruses, in which it appears to play a role in membrane penetration and cell-to-cell fusion. We sought to express BHV-1 open reading frame U(L)10, which encodes gM, and specifically identify the glycoprotein. We corrected a frameshift error in the published sequence and used the corrected sequence to design coterminal peptides from the C terminus. These were expressed as glutathione S-transferase fusion proteins in Escherichia coli. The fusion protein containing the 63 C-terminal amino acids from the corrected gM sequence engendered antibodies that immunoprecipitated a 30-kDa protein from in vitro translation reactions programmed with the U(L)10 gene. Proteins immunoprecipitated by this antibody from virus-infected cells ran at 36 and 43 kDa in reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and 43 and 48 kDa in nonreducing SDS-PAGE. Only the larger of the pair was present in virions. A 7-kDa protein was released from gM by reducing agents. The 7-kDa protein was not recognized in Western blots probed with the anti-gM ant...Continue Reading

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Citations

Jul 11, 2000·Journal of Virology·B G KluppT C Mettenleiter
Apr 12, 2001·The Journal of General Virology·B F KinghamC J Schmidt
Aug 9, 2005·Pflügers Archiv : European journal of physiology·Sandra Loch, Robert Tampé
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Jan 29, 2010·Journal of Virology·Tomohiko SadaokaYasuko Mori
Dec 17, 2014·Journal of Virology·Imane El Kasmi, Roger Lippé
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Feb 26, 2005·Journal of Virology·Janet S MayPhilip G Stevenson
Aug 13, 2015·The Journal of General Virology·Hannah StriebingerSusanne M Bailer
Mar 9, 2021·Frontiers in Microbiology·Chunmei LiBin Tian
Mar 23, 2010·Veterinary Microbiology·Bryan D GriffinEmmanuel J H J Wiertz

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