Bovine Herpesvirus-4-Vectored Delivery of Nipah Virus Glycoproteins Enhances T Cell Immunogenicity in Pigs.

Vaccines
Miriam PedreraGaetano Donofrio

Abstract

Nipah virus (NiV) is an emergent pathogen capable of causing acute respiratory illness and fatal encephalitis in pigs and humans. A high fatality rate and broad host tropism makes NiV a serious public and animal health concern. There is therefore an urgent need for a NiV vaccines to protect animals and humans. In this study we investigated the immunogenicity of bovine herpesvirus (BoHV-4) vectors expressing either NiV attachment (G) or fusion (F) glycoproteins, BoHV-4-A-CMV-NiV-GΔTK or BoHV-4-A-CMV-NiV-FΔTK, respectively in pigs. The vaccines were benchmarked against a canarypox (ALVAC) vector expressing NiV G, previously demonstrated to induce protective immunity in pigs. Both BoHV-4 vectors induced robust antigen-specific antibody responses. BoHV-4-A-CMV-NiV-GΔTK stimulated NiV-neutralizing antibody titers comparable to ALVAC NiV G and greater than those induced by BoHV-4-A-CMV-NiV-FΔTK. In contrast, only BoHV-4-A-CMV-NiV-FΔTK immunized pigs had antibodies capable of significantly neutralizing NiV G and F-mediated cell fusion. All three vectored vaccines evoked antigen-specific CD4 and CD8 T cell responses, which were particularly strong in BoHV-4-A-CMV-NiV-GΔTK immunized pigs and to a lesser extent BoHV-4-A-CMV-NiV-FΔTK. The...Continue Reading

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Citations

Oct 16, 2020·The Journal of General Virology·Nazia ThakurDalan Bailey
Jul 14, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Seval Bilge-DagalpGaetano Donofrio
Oct 2, 2021·Frontiers in Immunology·Daniel Rodríguez-MartínVerónica Martín

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Datasets Mentioned

BETA
AAK50554.1
AAK50553.1
AY816748.1
AY816746.1

Methods Mentioned

BETA
transfection
Protein Assay
electrophoresis
PCR
serum collection
lavage
density gradient centrifugation
ELISA
Assay

Software Mentioned

GraphPad Prism
GraphPad

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