Bovine herpesvirus 5 (BHV-5) Us9 is essential for BHV-5 neuropathogenesis

Journal of Virology
S I ChowdhuryY Zhou

Abstract

Bovine herpesvirus 5 (BHV-5) is a neurovirulent alphaherpesvirus that causes fatal encephalitis in calves. In a rabbit model, the virus invades the central nervous system (CNS) anterogradely from the olfactory mucosa following intranasal infection. In addition to glycoproteins E and I (gE and gI, respectively), Us9 and its homologue in alphaherpesviruses are necessary for the viral anterograde spread from the presynaptic to postsynaptic neurons. The BHV-5 Us9 gene sequence was determined, and the predicted amino acid sequence of BHV-5 Us9 was compared with the corresponding Us9 sequences of BHV-1.1. Alignment results showed that they share 77% identity and 83% similarity. BHV-5 Us9 peptide-specific antibody recognized a doublet of 17- and 19-kDa protein bands in BHV-5-infected cell lysates and in purified virions. To determine the role of the BHV-5 Us9 gene in BHV-5 neuropathogenesis, a BHV-5 Us9 deletion recombinant was generated and its neurovirulence and neuroinvasive properties were compared with those of a Us9 rescue mutant of BHV-5 in a rabbit model. Following intranasal infection, the Us9 rescue mutant of BHV-5 displayed a wild-type level of neurovirulence and neural spread in the olfactory pathway, but the Us9 deletion ...Continue Reading

References

Sep 2, 1985·FEBS Letters·G W WellingS Welling-Wester
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Nov 1, 1995·Journal of Virology·K S DingwellD C Johnson
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·M E PenfoldA L Cunningham
May 1, 1994·Veterinary Pathology·E B BelknapP C Schultheiss
Jan 1, 1997·Nucleic Acids Research·A BairochK Hofmann
Nov 5, 1997·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S E AshbaughJ K Collins
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Brandimarti, B Roizman
Mar 21, 1998·Journal of Comparative Pathology·S I ChowdhuryM L Weiss
Jan 19, 1999·Advances in Virus Research·L W EnquistG A Smith
Jun 26, 2001·Traffic·M J TomishimaL W Enquist
Aug 15, 2001·The Journal of Cell Biology·M J Tomishima, L W Enquist

❮ Previous
Next ❯

Citations

Jul 23, 2005·Journal of Neurovirology·Isamu MoriYoshinobu Kimura
Sep 13, 2003·Journal of Virology·G DelhonD L Rock
Jun 10, 2011·Acta Veterinaria Scandinavica·María F LadelfaSonia A Romera
Aug 31, 2014·Research in Veterinary Science·M S MarinS E Pérez
May 5, 2009·The Veterinary Journal·María P Del Médico ZajacSonia A Romera
Apr 29, 2005·Comparative Immunology, Microbiology and Infectious Diseases·S O HübnerP M Roehe
Nov 28, 2006·Journal of Virological Methods·Daniela Almeida Freitas AfonsoEdel Figueiredo Barbosa-Stancioli
Dec 6, 2005·Veterinary Microbiology·T H Ch'ng, L W Enquist
Sep 24, 2010·The Journal of General Virology·Alexandra NegatschWalter Fuchs
Apr 9, 2016·ACS Chemical Neuroscience·Douglas M DurrantRobyn S Klein
Mar 10, 2010·Veterinary Microbiology·Florian SteinerMartin Schwyzer
Aug 29, 2016·Archives of Virology·Sita Awasthi, Harvey M Friedman
Sep 24, 2016·Archives of Virology·Carolina Silvestro, Ana Bratanich
Dec 15, 2015·Journal of Virology·Russell J DiefenbachAnthony L Cunningham

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.