PMID: 6171499Nov 1, 1981Paper

Bovine hypothalamic poly(A)-rich RNA-directed synthesis of a common precursor to the nonapeptide arginine vasopressin and neurophysin II. Immunological identification and tryptic peptide mapping

Hoppe-Seyler's Zeitschrift für physiologische Chemie
H Schmale, D Richter

Abstract

The common precursor to arginine vasopressin (AVP) and neurophysin II (NpII) has been synthesized in a reticulocyte lysate system directed by bovine hypothalamic poly(A)-rich RNA. The precursor, identified with antibodies raised against neurophysin II and arginine vasopressin, has an apparent Mr of 21 000 (21 k). The specificity of the immune reaction has been shown by competition experiments using excess amounts of a variety of unlabeled peptides. With anti Np II, but not with anti AVP, a second neurophysin precursor with an apparent Mr of 18 000 (18 k) has been identified. Comparison of the tryptic maps obtained from the 21 k and 18 k precursors shows that both products give rise to the four [35S]cysteine-labeled neurophysin II-peptide fragments; however, only the 21 k yields an AVP-like tryptic peptide, identified with antibodies raised against AVP. The possible implications of the two precursors, one consisting of AVP and Np II, the other of Np II only, are discussed.

References

Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L C Giudice, I M Chaiken
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J T RussellH Gainer
May 1, 1977·The Journal of Cell Biology·H GainerM J Brownstein
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R H GoodmanJ F Habener
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P NicolasP Cohen
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H Schmale, D Richter

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