Bovine lactoferrin potently inhibits liver mitochondrial 8-OHdG levels and retrieves hepatic OGG1 activities in Long-Evans Cinnamon rats

Journal of Hepatology
Akihito TsubotaKiyotaka Fujise

Abstract

To assess the effect of lactoferrin on oxidative liver damage and its mechanism, we used Long-Evans Cinnamon (LEC) rats that spontaneously develop fulminant-like hepatitis and lethal hepatic failure. Four-week-old female LEC rats were divided into the untreated and treated groups. The latter was fed bovine lactoferrin at 2% mixed with conventional diet. The cumulative survival rates were 75.0% vs. 100% at 14 weeks, 37.5% vs. 91.7% at 15 weeks, and 12.5% vs. 91.7% at 16 weeks, respectively, for untreated and treated rats (P=0.0008). The 8-OHdG levels in liver mitochondrial DNA and malondialdehyde in plasma and liver tissues were significantly lower in treated than untreated rats (P<0.001, =0.017 and 0.034, respectively). Mitochondrial DNA mutations were more common in untreated rats. OGG1 mRNA and protein expression levels were significantly lower in untreated than treated rats (P=0.003 and 0.007, respectively). Hypermethylation of the second CpG island located upstream of OGG1 gene was observed in untreated rats. Our findings indicated that lactoferrin inhibits oxidative liver damage in LEC rats. Lactoferrin could be potentially useful for the treatment of oxidative stress-induced liver diseases.

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Citations

Apr 6, 2012·PloS One·Motoko KawashimaKazuo Tsubota
Sep 11, 2012·Journal of Clinical Biochemistry and Nutrition·Yasumasa OkazakiShinya Toyokuni
Mar 7, 2012·International Journal of Biological Sciences·Cui-Li ZhangKe-Qin Xie
Nov 30, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Yuki ShigaAtsushi Sato
Mar 17, 2016·Antioxidants & Redox Signaling·Sylvain MayeurEmile Levy
Dec 5, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kentaro ToriumiSusumu Takekoshi
Feb 5, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·G Y DenizF Geyikoglu
Mar 6, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zhigang WangZhenyuan Song
Oct 13, 2012·The Journal of Veterinary Medical Science·Yumi KimotoTakashi Takeuchi
Aug 2, 2017·Molecular Pharmaceutics·Yuki ShigaAtsushi Sato

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