PMID: 2125822Dec 1, 1990Paper

Bovine lens aldose reductase: tight binding of the pyridine coenzyme

Archives of Biochemistry and Biophysics
A Del CorsoU Mura

Abstract

Analysis by HPLC of the protein-free supernatant obtained after denaturation of aldose reductase shows that the native form of the enzyme (ARb) contains a tightly bound NADP+, which is absent in the oxidatively modified form (ARa). The absorption, fluorescence, and circular dichroism spectra of ARb and ARa are consistent with the presence of the cofactor only in the native form of aldose reductase. On the other hand, the modified enzyme, in appropriate thiol reducing conditions, can tightly bind NADP+. This indicates a potential reversibility of the modification of aldose reductase, at least in terms of retention of the cofactor.

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Citations

Feb 14, 2002·Archives of Biochemistry and Biophysics·Antonella Del CorsoUmberto Mura
Aug 7, 1993·Biochimica Et Biophysica Acta·S Q LiuS K Srivastava
Apr 13, 1994·Biochimica Et Biophysica Acta·A BhatnagarS K Srivastava
Feb 17, 1993·Biochimica Et Biophysica Acta·A FerrarettoS Ronchi
Oct 24, 2001·European Journal of Medicinal Chemistry·L CostantinoU Mura
Mar 12, 2002·Bioorganic & Medicinal Chemistry·Giulio RastelliSara Pacchioni
Jul 7, 2005·Antioxidants & Redox Signaling·Massimo Dal MonteUmberto Mura
Sep 11, 2013·PloS One·Antonella Del-CorsoUmberto Mura
Oct 1, 1992·Biochemical Medicine and Metabolic Biology·A Bhatnagar, S K Srivastava
Apr 18, 2001·Chemico-biological Interactions·M CappielloU Mura
Jul 29, 2015·Biochimica Et Biophysica Acta·Francesco BalestriUmberto Mura
Mar 4, 2014·Insect Molecular Biology·H GuoC-Z Wang
Nov 5, 1999·Experimental Eye Research·S BanditelliU Mura
Dec 22, 2014·Biochemical and Biophysical Research Communications·Francesco BalestriAntonella Del-Corso
Dec 27, 1996·The Journal of Biological Chemistry·M CappielloU Mura
Nov 25, 2019·Biochemical and Biophysical Research Communications·Vito BarraccoAntonella Del-Corso
Mar 21, 1998·Archives of Biochemistry and Biophysics·A Del CorsoU Mura
Apr 18, 2006·Archives of Biochemistry and Biophysics·Roberta MoschiniUmberto Mura
Aug 13, 2021·Protein Science : a Publication of the Protein Society·Paula MonchiettiDaniela L Catalano-Dupuy

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