journal cover

Bovine liver dihydrofolate reductase: purification and properties of the enzyme

Biochemistry

Dec 2, 1975

D L PetersonR L Blakley

Abstract

A purification procedure is reported for obtaining bovine liver dihydrofolate reductase in high yield and amounts of 100-200 mg. A key step in the procedure is the use of an affinity gel prepared by coupling pteroyl-L-lysine to Sepharose. The purified reductase has a specific activity o...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Immune Sera
Isoenzymes
Liver
Lysine
Purification Aspects
Methotrexate
Antigen-Antibody Reactions
Oxidoreductase
Alloenzymes
38
1
Paper Details
References
  • References
  • Citations26
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations26
123

Bovine liver dihydrofolate reductase: purification and properties of the enzyme

Biochemistry

Dec 2, 1975

D L PetersonR L Blakley

PMID: 45

DOI: 10.1021/bi00695a005

Abstract

A purification procedure is reported for obtaining bovine liver dihydrofolate reductase in high yield and amounts of 100-200 mg. A key step in the procedure is the use of an affinity gel prepared by coupling pteroyl-L-lysine to Sepharose. The purified reductase has a specific activity o...read more

Mentioned in this Paper

Hydrogen-Ion Concentration
Immune Sera
Isoenzymes
Liver
Lysine
Purification Aspects
Methotrexate
Antigen-Antibody Reactions
Oxidoreductase
Alloenzymes
38
1

Related Papers

Paper Details
References
  • References
  • Citations26
  • finger pointing at paper

    References currently unavailable

    We're still populating references for this paper, please check back later.
  • References
  • Citations26
123
/papers/bovine-liver-dihydrofolate-reductase-purification/45