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Bovine liver dihydrofolate reductase: purification and properties of the enzyme

Biochemistry

Dec 2, 1975

Darrell L PetersonR L Blakley

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Abstract

A purification procedure is reported for obtaining bovine liver dihydrofolate reductase in high yield and amounts of 100-200 mg. A key step in the procedure is the use of an affinity gel prepared by coupling pteroyl-L-lysine to Sepharose. The purified reductase has a specific activity o...read more

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Amino Acid [EPC]
Enterococcus faecium
Lysine
Homologous Gene
Antigen-Antibody Reactions
Vertebrates
Alloenzymes
Isoenzymes
Immune Sera
Metazoa
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  • Bovine liver dihydrofolate reductase: purification and properties of the enzyme

    Biochemistry

    Dec 2, 1975

    Darrell L PetersonR L Blakley

    PMID: 45

    DOI: 10.1021/bi00695a005

    Abstract

    A purification procedure is reported for obtaining bovine liver dihydrofolate reductase in high yield and amounts of 100-200 mg. A key step in the procedure is the use of an affinity gel prepared by coupling pteroyl-L-lysine to Sepharose. The purified reductase has a specific activity o...read more

    Mentioned in this Paper

    Amino Acid [EPC]
    Enterococcus faecium
    Lysine
    Homologous Gene
    Antigen-Antibody Reactions

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