PMID: 9533637Apr 9, 1998Paper

Bovine milk inhibits proteolytic degradation of epidermal growth factor in human gastric and duodenal lumen

Peptides
R K RaoS S Baker

Abstract

Degradation of epidermal growth factor (EGF) in human gastric and duodenal lumen was analyzed by incubating 125I-labeled or unlabeled human recombinant EGF with human gastric or duodenal luminal fluids in vitro. Degradation of EGF was assessed by measuring the generation of acid soluble radioactivity or by reversed-phase high-performance liquid chromatography (HPLC). Incubation with gastric luminal fluids resulted in a time- and dose-dependent degradation of labeled and unlabeled EGF at pH 2.5 but not at pH 7.5. Duodenal luminal fluids, on the other hand, degraded EGF at pH 7.5 but not at pH 2.5. The rate of degradation of unlabeled EGF in gastric luminal fluids was nearly 12-fold higher than the rate of degradation of labeled EGF, whereas only a slight difference in rates of degradation of labeled and unlabeled EGF was observed in duodenal luminal fluids. High-performance liquid chromatography analysis detected three major degradation products that eluted with retention time of 17.5 min, 20.0 min, and 22.5 min that was associated with a reduction of intact EGF (retention time 23.5 min). Defatted and decaseinated supernatant of bovine milk effectively inhibited the degradation of EGF in both gastric and duodenal luminal fluids....Continue Reading

References

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Citations

Jan 21, 2015·Critical Reviews in Biotechnology·Russell G A Jones, Angela Martino
Jul 19, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·José Moisés LaparraRosaura Farré
Sep 14, 1999·JPEN. Journal of Parenteral and Enteral Nutrition·W M Wong, N A Wright

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