Abstract
Bovine neonatal pancytopenia (BNP) was a vaccine-induced alloimmune disease observed in young calves and characterized by hemorrhages, pancytopenia, and severe destruction of the hematopoietic tissues. BNP was induced by alloreactive maternal antibodies present in the colostrum of certain cows vaccinated with a highly adjuvanted vaccine against bovine viral diarrhea. Bioprocess impurities, originating from the production cell line of the vaccine, are likely to have induced these alloreactive antibodies. One prominent alloantigen recognized by vaccine-induced alloantibodies is highly polymorphic bovine major histocompatibility complex class I antigen (bovine leukocyte antigen 1-BoLA I). Aim of this study was to define the fine specificity of BNP-associated anti-BoLA I alloantibodies. In total, eight different BoLA I alleles from the production cell line were identified. All genes were cloned and recombinantly expressed in murine cell lines. Using these cells in a flow cytometric assay, the presence of BoLA I specific alloantibodies in BNP dam sera was proven. Three BoLA I variants were identified that accounted for the majority of vaccine-induced BoLA I reactivity. By comparing the sequence of immunogenic to non-immunogenic BoLA...Continue Reading
References
Nov 1, 1993·The Journal of Clinical Investigation·S SantosoP J Newman
Mar 27, 1997·Transplantation·G E RodeyT C Fuller
Mar 21, 1998·Electrophoresis·N Guex, M C Peitsch
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Jul 1, 1958·Proceedings of the Society for Experimental Biology and Medicine·S H MADIN, N B DARBY
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Feb 24, 2006·Transplantation·Jagdeep Obhrai, Daniel R Goldstein
Jan 9, 2007·Immunogenetics·Shawn BabiukReno Pontarollo
Apr 24, 2008·Immunogenetics·James BirchShirley A Ellis
Mar 6, 2010·Transboundary and Emerging Diseases·B PardonP Deprez
Jan 26, 2011·The Veterinary Record·C R BellC D Penny
Jan 29, 2011·Veterinary Immunology and Immunopathology·Philip Simon BridgerKlaus Doll
Feb 22, 2011·BMC Veterinary Research·Annette FriedrichCarola M Sauter-Louis
May 25, 2011·Vaccine·Max BastianKlaus Cussler
Sep 1, 2011·Veterinary Research·Fabian DeutskensTill Rümenapf
Nov 16, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gilles FoucrasFrançois Schelcher
Mar 6, 2012·Preventive Veterinary Medicine·Carola Sauter-LouisWolfgang Klee
May 19, 2012·PloS One·Sarah L LambtonAndrew Holliman
Sep 11, 2012·Vaccine·Rahel KasontaMax Bastian
Jan 25, 2013·BMC Veterinary Research·Kerstin N EulerCornelia A Deeg
Jul 13, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melanie AllesJörg Kirberg
Nov 11, 2014·Annual Review of Animal Biosciences·Shirley A Ellis, John A Hammond
Dec 18, 2014·Veterinary Research·Lindert BenedictusAd P Koets
Aug 4, 2015·Scientific Reports·Lindert BenedictusAd P Koets