PMID: 6161221Aug 1, 1980Paper

Bovine peripheral nervous system myelin P2 protein: chemical and immunological characterization of the cyanogen bromide peptides

Journal of Neurochemistry
M J WeiseS Brostoff

Abstract

Cleavage of bovine P2 protein by cyanogen bromide (CNBr) produced peptide fractions CN1, CN2, and CN3 which were isolated by gel filtration chromatography. CN2 was found to contain two NH2-terminals (lysine and valine) and accounted for both of the cysteine residues of P2. When reduced carboxymethylated P2 (RCM-P2) was digested with CNBr, peptides CN1 and CN3 were obtained as were (1) a peptide with NH2-terminal lysine (Lys) that contained no homoserine and only one cysteine residue and (2) a peptide with NH2-terminal valine (Val) that was co-eluted with CN3. These data and the chemical characterization of all the CNBr peptides obtained from P2 and RCM-P2 suggest that isolated P2 protein has a structure composed of the CNBr peptides in the order CN3-CN1-CN2(Val)-CN2(Lys) with an intrachain disulfide bond between the cysteine residues located in the two constituent peptides of CN2, CN2(Lys) and CN2(Val). To locate the neuritogenic region(s) within the P2 protein structure, CN1, CN2, and CN3 were tested for the ability to induced experimental allergic neuritis (EAN) in Lewis rats. The disease-inducing sites of P2 protein were found only in CN1; neither CN2 nor CN3 produced disease. EAN induced by CN1 was comparable to that induce...Continue Reading

References

Feb 1, 1975·Journal of Neurochemistry·S W BrostoffC DiPaola
Jan 11, 1979·Nature·M Kadlubowski, R A Hughes
Nov 25, 1977·Biochimica Et Biophysica Acta·A IshaqueE H Eylar
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Citations

Aug 16, 2000·Journal of Colloid and Interface Science·C LopezM Ollivon
May 1, 1986·Lipids·K C LeskawaS W Brostoff
Jan 10, 1983·Brain Research·J M CunninghamS W Brostoff
Nov 20, 1987·Journal of Molecular Biology·T BergforsM Weise
Jun 17, 2000·Biochimica Et Biophysica Acta·J Storch, A E Thumser
Aug 1, 1988·Journal of Neuroimmunology·T OleeS W Brostoff
Oct 1, 1984·Neurochemical Research·J N Whitaker, J M Seyer
Oct 1, 1984·Neurochemical Research·K UyemuraK Kitamura
Aug 1, 1980·Journal of Neurochemistry·M J WeiseS W Brostoff
Sep 1, 1982·Journal of Neurochemistry·K UyemuraI Muramatsu
Jun 1, 1988·Journal of Neurochemistry·J SedzikM Weise
Jan 1, 1981·Annals of Neurology·J N Whitaker

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