PMID: 41711Oct 15, 1979

Bovine serum chitinase

European Journal of Biochemistry
G LundbladK Slettengren

Abstract

1. A glycol-chitin-splitting enzyme without lysozyme (muramidase) activity has been found in calf serum. The enzyme also degrades colloidal chitin and is thus a true chitinase, 1,4-beta-poly-N-acetylglucosaminidase, without exo-beta-N-acetylglucosaminidase effect. 2. The enzyme is purified 1000-fold by ion-exchange chromatography and gel filtration. Its optimal activity is between pH 1.5-2.0 with glycol chitin and between pH 3-6 in a rather broad optimum with colloidal chitin as substrate. The optimal stability of the enzyme is in the pH interval 3.0-6.5 when tested by incubation with glycol chitin at 50 degrees C for 60 min. The optimal temperature for the degradation of glycol chitin is 40 degrees C when assayed at pH 1.5 and 51 degrees C when assayed at pH 3.5. 3. The enzyme is activated by moderate heating at pH 6.5. The highest relative activity, 135% is reached after 45 min incubation at 30 degrees C, pH 5 or after 30 min at 40, pH 2.4. By incubation with small amounts of trypsin at pH 6.5 at 3m degrees C the enzyme was temporarily activated. 4. The isoelectric point, pH 5.3, and the molecular weight, 47,000 +/- 3,000 were determined by respectively isoelectric focusing and gel filtration. 5. The Michaelis-Menten constant...Continue Reading

References

Jan 1, 1991·Archives of Insect Biochemistry and Physiology·U Sommer, K D Spindler
Jun 1, 1996·Molecular and Biochemical Parasitology·A VenegasJ A Fuhrman
Jun 1, 1982·Archives of Biochemistry and Biophysics·A C ChenJ R DeLoach
Oct 10, 2001·FEBS Letters·M SuzukiB Syuto

Citations

Jan 1, 1976·Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry·G LundbladJ Lind
Jul 15, 1974·European Journal of Biochemistry·G LundbladM Steby
Jan 1, 1966·Scandinavian Journal of Clinical and Laboratory Investigation·G Lundblad, E Hultin
May 1, 1966·Canadian Journal of Biochemistry·H TakedaC Roy
Nov 1, 1956·Archives internationales de physiologie et de biochimie·C JEUNIAUX
Sep 1, 1958·Biochimica Et Biophysica Acta·L R BERGER, D M REYNOLDS
May 1, 1963·Journal of Biochemistry·K HAYASHIM FUNATSU
Jul 1, 1963·Canadian Journal of Biochemistry and Physiology·G A STRADINE, D R WHITAKER

Related Concepts

Endochitinase
CHIT1
Drug Stability
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Hydrogen-Ion Concentration
Enzyme Activation

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