Bovine sperm raft membrane associated Glioma Pathogenesis-Related 1-like protein 1 (GliPr1L1) is modified during the epididymal transit and is potentially involved in sperm binding to the zona pellucida

Journal of Cellular Physiology
Julieta CaballeroR Sullivan

Abstract

Glioma pathogenesis-related 1-like protein1 (GliPr1L1) was identified by liquid chromatography-tandem mass spectrometry analyses of proteins associated to bovine sperm lipid raft membrane domains. This protein belongs to the CAP superfamily including cysteine-rich secretory proteins, Antigen 5 and pathogenesis-related 1 protein. PCR analysis revealed that GliPr1L1 is expressed in testis and, at a much lower level, all along the epididymis. Western blotting showed a similar distribution of GliPr1L1 in testicular and epididymal tissue extracts. In the epididymal lumen, GliPr1L1 was associated with the maturing spermatozoa and epididymosomes all along the excurrent duct but was undetectable in the soluble fraction of epididymal fluid. The protein was detectable as multiple isoforms with a higher MW form in the testis and proximal caput. Treatments with PNGase F revealed that N-glycosylation was responsible of multiple bands detected on Western blots. These results suggest that the N-glycosylation moiety of GliPr1L1 is processed during the transit in the caput. Western blots demonstrated that GliPr1L1 was associated with the sperm plasma membrane preparation. GliPr1L1 is glycosyl phosphatidyl inositol (GPI) anchored to caput and ca...Continue Reading

References

Nov 26, 1999·Biology of Reproduction·R ShamsadinW Engel
Jun 25, 2004·Biology of Reproduction·Nicholas L Cross
May 17, 2005·Blood Cells, Molecules & Diseases·Robert SullivanGilles Frenette
Feb 20, 2007·Gene·Mahnaz Ekhlasi-HundrieserEdda Töpfer-Petersen
Jun 26, 2007·Asian Journal of Andrology·Robert SullivanJulie Girouard
Jun 26, 2007·Asian Journal of Andrology·Kenneth P RobertsDavid W Hamilton
Sep 4, 2007·Seminars in Cell & Developmental Biology·Prabuddha SenguptaDavid Holowka
Jul 24, 2008·The International Journal of Developmental Biology·Bart M GadellaIan A Brewis
Jul 24, 2008·The International Journal of Developmental Biology·Débora J CohenPatricia S Cuasnicu
Aug 30, 2008·Journal of Cellular Physiology·Brett NixonR John Aitken
Nov 14, 2008·Theriogenology·P BlondinW A King
Jan 13, 2009·Human Reproduction Update·Gail A Cornwall
Oct 28, 2009·Journal of Reproductive Immunology·Brett Nixon, R John Aitken
Jun 4, 2010·The Journal of Physiology·Caroline Dart
Oct 23, 2010·Journal of Andrology·Yuko Muro, Masaru Okabe
Nov 3, 2010·Asian Journal of Andrology·Andrew T ReidBrett Nixon

❮ Previous
Next ❯

Citations

Apr 25, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Robert Sullivan, Fabrice Saez
Sep 24, 2015·Journal of Cellular Physiology·Olivier D'AmoursRobert Sullivan
May 1, 2015·Asian Journal of Andrology·Tamara Leahy, Bart M Gadella
Jun 24, 2016·Animal Reproduction Science·Maren GottschalkOttmar Distl
Oct 4, 2016·PloS One·Olivia JerczynskiClémence Belleannée
Feb 2, 2018·Endocrine Reviews·Carlos SimonFelipe Vilella
Oct 17, 2015·Clinical and Experimental Reproductive Medicine·Yong-Pil Cheon, Chung-Hoon Kim
Feb 23, 2020·Molecular Human Reproduction·A Vashisht, G K Gahlay
May 8, 2020·Human Reproduction Update·Avinash S GaikwadMoira K O'Bryan
Sep 26, 2020·Frontiers in Cell and Developmental Biology·Maíra Bianchi Rodrigues AlvesClémence Belleannée
Feb 27, 2021·Molecular Reproduction and Development·Birgitte NarudElisabeth Kommisrud
Sep 3, 2020·Journal of Proteome Research·Rongxia LanShisheng Sun
May 15, 2021·Journal of Animal Science and Technology·Sung Woo Kim, Bongki Kim
Jul 1, 2021·Journal of Extracellular Vesicles·Natalie J FootSharad Kumar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.