Bovine spongiform encephalopathy in Sweden: an H-type variant

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Dolores Gavier-WidenT Baron

Abstract

Bovine spongiform encephalopathy (BSE) had never been detected in Sweden until 2006, when the active surveillance identified a case in a 12-year-old cow. The case was an unusual form, because several molecular features of the protease-resistant prion protein (PrP(res)) were different from classical BSE. The differences included higher susceptibility for proteinase K, higher molecular weight of the PrP(res) bands, affinity to the N-terminus-specific antibodies 12B2 and P4, and peculiar banding pattern with antibody SAF84 showing an additional band at the 14 kDa position. The molecular characteristics were in accordance to previous descriptions of H-type BSE. This report shows that a range of Western blot techniques and antibodies can be applied to confirm H-type BSE and further describes that the ratio of the amounts of PrPres#1 and PrPres#2, after deglycosylation, depends on the antibody used during processing. Immunohistochemistry on sections of medulla at the level of the obex applying antibodies with epitopes covering a broad range of the PrP sequence showed accumulation of disease-specific PrP (PrP(d)) in the gray matter. Fine punctate deposition in the neuropil was the most predominant type and was more severe in BSE targe...Continue Reading

References

Oct 31, 1987·The Veterinary Record·G A WellsR Bradley
Mar 29, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M BruceH Fraser
Apr 6, 1996·Lancet·R G WillP G Smith
Jun 27, 1996·Nature·C I LasmézasJ J Hauw
Oct 23, 1997·Nature·A F HillP Lantos
Dec 22, 1999·Biochemical and Biophysical Research Communications·S DemartJ P Deslys
Sep 2, 2000·Journal of Clinical Microbiology·K I O'RourkeD P Knowles
Sep 6, 2003·The Veterinary Record·S L BenestadB Bratberg
Jan 8, 2004·EMBO Reports·Anne-Gaëlle BiacabeThierry Baron
Feb 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cristina CasaloneMaria Caramelli
Dec 25, 2004·The Journal of Biological Chemistry·Cécile FéraudetJacques Grassi
Mar 25, 2006·Australian Veterinary Journal·M P Hartley
Jun 1, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stéphane LezmiThierry Baron
Jun 9, 2006·The Journal of General Virology·Michael StackDanny Matthews
Jun 13, 2006·BMC Veterinary Research·Jan P M LangeveldLucien J M van Keulen
Jul 14, 2006·Emerging Infectious Diseases·Thierry G M BaronJan P M Langeveld
Aug 19, 2006·Veterinary Microbiology·A BuschmannMartin H Groschup
Mar 1, 2007·Emerging Infectious Diseases·Torsten SeuberlichAndreas Zurbriggen
Apr 4, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jürgen A RichtS Mark Hall

❮ Previous
Next ❯

Citations

Jan 6, 2009·Journal of the American Veterinary Medical Association·Jane L Harman, Christopher J Silva
Oct 14, 2008·Pathologie-biologie·H Laude, V Béringue
Jun 6, 2009·Zoonoses and Public Health·M StackL Terry
Jan 30, 2015·Prion·Leonor OrgeJ Pedro Simas
Apr 25, 2009·Acta Microbiologica Et Immunologica Hungarica·M Füzi, P Horváth
Nov 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Torsten SeuberlichAndreas Zurbriggen
Jun 4, 2008·Veterinary Research·Vincent BéringueHubert Laude
Dec 20, 2012·The Veterinary Record·M J StackP Burke

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
genotyping
ELISA
electron microscopy
transgenic

Software Mentioned

Quantity One

Related Concepts

Related Feeds

Bovine Spongiform Encephalopathy

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.

Bovine Spongiform Encephalopathy (MDS)

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
J A RichtS Mark Hall
Proceedings of the National Academy of Sciences of the United States of America
Cristina CasaloneMaria Caramelli
© 2021 Meta ULC. All rights reserved