PMID: 7538798Mar 1, 1995Paper

Bovine SRY gene locus: cloning and testicular expression

Biology of Reproduction
Isabelle DaneauDavid W Silversides

Abstract

The bovine SRY gene was cloned by a combination of anchored polymerase chain reaction (PCR) amplification of genomic restriction fragments and reverse transcription-PCR (RT-PCR) of testicular RNA. We report 1800 bp of combined genomic and cDNA sequences including 911 bp of 5' upstream sequences, an open reading frame of 687 bp, and 202 bp of sequences corresponding to the 3' end of the mRNA. The bovine SRY gene encodes a deduced (predicted on the basis of a cDNA sequence) protein product of 229 amino acids, with sequence conservation between species, notably in the region of the high-mobility group (HMG) domain or HMG box. Outside of the HMG box, the bovine SRY structure shows greater resemblance to the human SRY than to the mouse Sry. As with human SRY promoter sequences, putative binding sites for Sp1 and for SRY itself are seen in the bovine SRY promoter region. Unlike the human SRY promoter, CAAT and TATA box motifs are present in the bovine sequences. Southern analysis and PCR amplification of male and female bovine genomic DNA show that the described sequences are specific to the Y chromosome. Northern analysis of bull testicular RNA demonstrated low levels of expression of the bovine SRY gene in adult testes with a major...Continue Reading

Citations

Jul 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·T VogelF Schnieders
Feb 24, 2001·Theriogenology·D W SilversidesJ Lussier
Jun 2, 2001·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·P Garcia-MeunierC Guinet
Apr 23, 2009·Reproduction, Fertility, and Development·F GeorgeI Donnay
Jul 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ti-Cheng ChangWan-Sheng Liu
Feb 28, 2003·Biology of Reproduction·Nicolas PilonDavid W Silversides
Oct 15, 2008·BMC Molecular Biology·Diana G F RossSigrid Lehnert
Dec 17, 2009·The Journal of Veterinary Medical Science·Chiho MoriyamaShunichi Kamimura
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A M WallaceC C MacDonald
Jun 1, 1996·Current Opinion in Genetics & Development·Y Ramkissoon, P Goodfellow
Sep 27, 2005·Biochemical and Biophysical Research Communications·Masanori ItoHideaki Tojo
Oct 8, 2016·Animal Biotechnology·Bijan SoleymaniAli Mostafaie
Aug 31, 2002·Genesis : the Journal of Genetics and Development·Isabelle DaneauDavid W Silversides
Jan 18, 2018·BMC Evolutionary Biology·Yukako KatsuraYoko Satta
Feb 23, 1999·The Journal of Veterinary Medical Science·T HasegawaH Mukoyama
Feb 11, 1998·Physiological Reviews·C M Haqq, P K Donahoe
Sep 10, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Pietro ParmaGiuseppe Enne
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Marcello RubessaMatthew B Wheeler
Oct 1, 2020·Scientific Reports·Joseph R OwenAlison L Van Eenennaam
Jun 17, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Monte E TurnerAmy Milsted
May 8, 1998·Biochemical and Biophysical Research Communications·E MargaritR Oliva
Jun 19, 1998·Biochimica Et Biophysica Acta·M DesclozeauxB Boizet-Bonhoure
May 29, 2021·Cellular and Molecular Life Sciences : CMLS·Ming WangChangxin Wu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.