Bovine TWINKLE and mitochondrial ribosomal protein L43 genes are regulated by an evolutionary conserved bidirectional promoter

Gene
Cédric MeerssemanDominique Rocha

Abstract

TWINKLE is a mitochondrial DNA helicase playing an important role in mitochondrial DNA replication. In human, mutations in this gene cause progressive external ophtalmoplegia and mitochondrial DNA depletion syndrome-7. TWINKLE is well conserved among multicellular eukaryotes and is believed to be a key regulator of mitochondrial DNA copy number in mammals. Despite its involvement in several diseases and its important function in mitochondrial DNA metabolism, nothing is known about the regulation of the expression of TWINKLE. We have analysed the 5'-flanking genomic region of the bovine TWINKLE gene and found it was localised adjacent to the MRPL43 gene in a head-to-head orientation, suggesting that both genes are regulated by a shared bidirectional promoter. The bovine 75-bp long intergenic region shows substantial homology across different species and contains several conserved putative transcription factor binding sites. A TATA box, however, was lacking. Using a dual fluorescent reporter system and transient transfection assays, we have analysed the bovine intergenic region between TWINKLE and MRPL43. This small genomic fragment showed a bidirectional promoter activity. As the TWINKLE/MRPL43 bidirectional promoter tested was ...Continue Reading

References

Feb 12, 1998·Biochemical and Biophysical Research Communications·G SchmiedeknechtG Schmitz
Sep 12, 2001·The Journal of Biological Chemistry·J K KumarC C Richardson
Mar 14, 2002·Biochemical and Biophysical Research Communications·A G MuellerA Schürmann
Dec 9, 2003·Molecular Biology and Evolution·Daiya Takai, Peter A Jones
Jan 7, 2004·Genome Research·Nathan D TrinkleinRichard M Myers
May 29, 2004·The EMBO Journal·Jenny A KorhonenMaria Falkenberg
Oct 29, 2004·Human Molecular Genetics·Henna TyynismaaAnu Suomalainen
Feb 23, 2007·Genes and Immunity·G E DaviesS K Anderson
Nov 28, 2007·Nucleic Acids Research·Géraldine FargeMaria Falkenberg
Feb 19, 2008·Journal of Molecular Biology·Jenny A KorhonenMaria Falkenberg
Oct 10, 2008·BMC Genomics·Mary Qu Yang, Laura L Elnitski
Mar 3, 2012·The Journal of Biological Chemistry·Doyel SenSmita S Patel

❮ Previous
Next ❯

Citations

Jun 16, 2016·Frontiers in Plant Science·Rui WangYongjun Lin
Mar 25, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Cédric MeerssemanDominique Rocha

❮ Previous
Next ❯

Related Concepts

Related Feeds

Ataxias (MDS)

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxia

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on ataxia here.

Ataxias

Ataxia is a neurological condition characterized by lack of voluntary coordination of muscle movements including loss of coordination, balance, and speech. Discover the latest research on different types of ataxias here.

Bovine Spongiform Encephalopathy (MDS)

Bovine spongiform encephalopathy is a neurodegenerative disease belonging to the transmissible spongiform encephalopathies, a group of diseases including sheep scrapie and human Creutzfeldt-Jakob disease. Here is the latest research.