Bovine whey fractionation based on cation-exchange chromatography

Journal of Chromatography. a
R HahnAlois Jungbauer

Abstract

Bovine whey proteins have potential applications in veterinary medicine, food industry and as supplements for cell culture media. A fractionation scheme for the economically interesting proteins, such as IgG, lactoferrin and lactoperoxidase, based on cation exchangers was the goal of our investigations. A chromatographic process was developed where alpha-lactalbumin passes through the column and separation of the desired proteins is achieved. Four different cation-exchange media (S-HyperD-F, S-Sepharose FF, Fractogel EMD SO3- 650 (S) and Macro-Prep High S Support) were compared in regard to their dynamic binding capacity for IgG and their different elution behaviours when sequential step gradients with NaCl buffers were applied. Peak fractions were analyzed by size-exclusion chromatography and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Lactoperoxidase activity was monitored by the oxidation of o-phenylenediamine. In order to explain the different resolution behaviours, isocratic runs with pure standards of whey proteins were performed. The k' values were calculated and plotted against salt concentration. Fractogel EMD had the highest binding capacity for IgG, 3.7 mg/ml gel at a linear flow-rate of 100 cm/h, but...Continue Reading

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Citations

Mar 23, 2011·Biotechnology Letters·Mayyada M H El-Sayed, Howard A Chase
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Linda PedersenAlois Jungbauer
Oct 22, 2011·International Journal of Environmental Research and Public Health·Yafei LiangWanping Zhu
Apr 17, 2010·Journal of Pharmaceutical and Biomedical Analysis·Luigi CampanellaMauro Tomassetti
Aug 14, 2012·Biotechnology Journal·Beckley K NforMarcel Ottens
Apr 6, 2017·Biotechnology Progress·Alexander MatlschweigerRainer Hahn
May 28, 2003·Biotechnology and Bioengineering·Beelin Cheang, Andrew L Zydney
Jun 5, 2003·Journal of Dairy Science·Chia-Kai Su, Been Huang Chiang
Oct 29, 2005·Biotechnology and Bioengineering·Gautam Lal BaruahGeorges Belfort
Jan 17, 2021·Journal of Chromatography. a·Linling Yu, Yan Sun
Dec 24, 2020·International Journal of Molecular Sciences·Adrian GołębiowskiBogusław Buszewski
Jun 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·B SchlattererK Schlatterer
Jul 9, 2021·Frontiers in Nutrition·Ayşenur ArslanSercan Karav
Dec 13, 2019·Journal of Agricultural and Food Chemistry·Rivca L Valk-WeeberSander S van Leeuwen

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