Bovis Bacillus Calmette-Guerin (BCG) infection induces exosomal miRNA release by human macrophages

Journal of Translational Medicine
Shamila D AlipoorIan M Adcock

Abstract

Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30-100 nm vesicles produced by most cell types and are found in almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells and tissues and may act as potential biomarkers in many diseases. In this pilot study, we assessed the miRNA profile of exosomes released from human monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette-Guerin (BCG). Human monocytes were obtained from the peripheral blood of three healthy subjects and driven to a monocyte-derived macrophage (MDM) phenotype using standard protocols. MDMs were infected with BCG or left uninfected as control. 72 h post-infection, exosomes were collected from the cell culture medium, RNA was isolated and RNA-seq performed. The raw reads were filtered to eliminate adaptor and primer sequences and the sequences were run against the mature human miRNA sequences available in miRBase. MicroRNAs were identified using an E value <0.01. miRNA network analysis was performed using the DIANA miRNA tool,...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Sep 15, 2000·Journal of Immunological Methods·A K LehmannA Halstensen
Feb 6, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·J S FriedlandJ-M Dayer
Jul 15, 2003·Clinical Microbiology Reviews·Issar Smith
Apr 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sara BusettoRenzo Menegazzi
May 17, 2005·Nature Medicine·Ernesto J Muñoz-Elías, John D McKinney
Oct 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paul T G ElkingtonJon S Friedland
Jun 27, 2007·The Journal of Biological Chemistry·Sanchita Bhatnagar, Jeffrey S Schorey
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Amit K Pandey, Christopher M Sassetti
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Jul 23, 2008·Nature Immunology·David BaltimoreKonstantin D Taganov
Mar 18, 2009·Clinical Lung Cancer·Guilherme RabinowitsGoetz H Kloecker
May 5, 2009·Journal of Inflammation·Hadi SarirGert Folkerts
Jul 3, 2010·EMBO Molecular Medicine·Mi-Jeong KimDavid G Russell
Apr 2, 2011·Journal of Proteome Research·Ji-Hyun ShinGeum-Sook Hwang
May 13, 2011·Trends in Microbiology·Kyu Y RheeCarl Nathan
Dec 24, 2011·Current Opinion in Allergy and Clinical Immunology·Tracey L Bonfield, Kristie R Ross
Jan 31, 2012·Bioinformatics·Martin ReczkoArtemis G Hatzigeorgiou
Apr 19, 2012·Nature Communications·Kun WangPei-Feng Li
Jun 6, 2012·RNA Biology·Ana EulalioJörg Vogel
Oct 3, 2012·Cold Spring Harbor Protocols·Colin MaloneGregory J Hannon
Jan 12, 2013·The Journal of Biological Chemistry·Wonsik LeeDavid G Russell
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 19, 2013·Journal of Lipid Research·Kasey C VickersAlan T Remaley
May 18, 2013·Nucleic Acids Research·Maria D ParaskevopoulouA G Hatzigeorgiou
Jun 26, 2013·Cellular Microbiology·Cathy Staedel, Fabien Darfeuille
Jul 13, 2013·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
Aug 15, 2013·Molecular and Cellular Biochemistry·Ping LiGuojun Zhang
Aug 16, 2013·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Riccardo CazzoliHarvey I Pass
Aug 17, 2014·FEBS Letters·Claire MaudetAna Eulalio
Oct 9, 2014·Annual Review of Physiology·Stella Kourembanas
Oct 10, 2014·Current Respiratory Care Reports·Anna K PersonTimothy R Sterling
Nov 2, 2014·Scandinavian Journal of Immunology·M FrydrychowiczG Dworacki

❮ Previous
Next ❯

Citations

Feb 2, 2018·FEMS Microbiology Letters·Juan José González Plaza
Oct 15, 2018·Journal of Cellular Physiology·Shima HadifarFarzam Vaziri
Sep 13, 2019·International Journal of Mycobacteriology·Shamila D AlipoorEsmaeil Mortaz
Jan 15, 2020·International Reviews of Immunology·Yuntong LiuDongmei Yan
Oct 8, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Quincy A HathawayJohn M Hollander
Mar 20, 2019·Disease Markers·Shamila D AlipoorEsmaeil Mortaz
Nov 16, 2019·Signal Transduction and Targeted Therapy·Yang LiWeibing Yang
Jun 2, 2018·BioMed Research International·Esmaeil MortazIan M Adcock
Jan 8, 2021·Journal of Clinical Medicine·Fantahun BiadglegneUlrich Sack
Sep 14, 2020·European Journal of Pharmacology·Shamila D AlipoorEsmaeil Mortaz
Nov 13, 2020·Signal Transduction and Targeted Therapy·Yang LiWeibing Yang
May 1, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ewa Kosior-JareckaTomasz Żarnowski
May 19, 2021·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Mostafa AtashbastehAbdolamir Allameh
Jul 3, 2021·Biomedicines·Naveed AkbarRobin P Choudhury

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
electron microscopy
electrophoresis
transmission electron microscopy

Software Mentioned

DIANA miRNA tool
BLAST
Drosha

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.