Bowman-Birk proteinase inhibitor from Clitoria fairchildiana seeds: Isolation, biochemical properties and insecticidal potential

Phytochemistry
Miriam DantzgerMaria Lígia Rodrigues Macedo

Abstract

Herein described is the biochemical characterisation, including in vitro and in vivo assays, for a proteinase inhibitor purified from Clitoria fairchildiana seeds (CFPI). Purification was performed by hydrophobic interaction and gel filtration chromatography. Kinetic studies of the purified inhibitor showed a competitive-type inhibitory activity against bovine trypsin and chymotrypsin, with an inhibition stoichiometry of 1:1 for both enzymes. The inhibition constants against trypsin and chymotrypsin were 3.3 × 10(-10) and 1.5 × 10(-10)M, respectively, displaying a tight binding property. SDS-PAGE showed that CFPI has a single polypeptide chain with an apparent molecular mass of 15 kDa under non-reducing conditions. However, MALDI-TOF analysis demonstrated a molecular mass of 7.973 kDa, suggesting that CFPI is dimeric in solution. The N-terminal sequence of CFPI showed homology with members of the Bowman-Birk inhibitor family. CFPI remained stable to progressive heating for 30 min to each temperature range of 37 up to 100 °C and CD analysis exhibited no changes in spectra at 207 nm after heating at 90 °C and subsequent cooling. Moreover, CFPI was active over a wide pH range (2-10). In contrast, reduction with DTT resulted in a l...Continue Reading

References

Nov 1, 1979·Analytical Biochemistry·E G DelMarM C Geokas
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Jan 1, 1980·Annual Review of Biochemistry·M Laskowski, I Kato
May 1, 1996·Journal of Molecular Evolution·B PrakashL R Gowda
Aug 12, 1999·Biochemical and Biophysical Research Communications·N R BuenoM L Oliva
Aug 21, 2002·Biochemistry·Arnd B E BrauerRobin J Leatherbarrow
Sep 27, 2002·Biopolymers·Jeffrey D McBrideRobin J Leatherbarrow
Feb 26, 2004·Archives of Insect Biochemistry and Physiology·A R LopesW R Terra
Oct 31, 2007·Insect Biochemistry and Molecular Biology·Daniela BrioschiMarcio C Silva-Filho
Nov 21, 2007·Journal of Agricultural and Food Chemistry·Railene de Azevedo PereiraMaria Fátima Grossi-de-Sá
Nov 11, 2008·Journal of Agricultural and Food Chemistry·Vanessa da Silveira RamosMaria Lígia Rodrigues Macedo
Nov 15, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Vanessa da Silveira RamosMaria Lígia Rodrigues Macedo
Feb 12, 2010·Journal of Agricultural and Food Chemistry·Elaprolu R PrasadK Padmasree
Mar 18, 2010·Archives of Insect Biochemistry and Physiology·Maria Lígia Rodrigues MacedoJosé Roberto Postali Parra
Dec 24, 2011·Nature·Ivan Semeniuk, Susan Young
Mar 7, 2012·Archives of Insect Biochemistry and Physiology·Marko PetekMaarten A Jongsma
Jun 9, 2012·Plant Physiology and Biochemistry : PPB·Domenico De PaolaGabriella Sonnante
Aug 14, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Vanessa da S RamosMaria L R Macedo
Mar 8, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Caio Fernando Ramalho de OliveiraMaria Ligia Rodrigues Macedo
Feb 4, 2014·Pesticide Biochemistry and Physiology·Caio Fernando Ramalho de OliveiraMaria Lígia Rodrigues Macedo

❮ Previous
Next ❯

Citations

Oct 19, 2019·Archives of Insect Biochemistry and Physiology·Eduardo G MendonçaMaria G de Almeida Oliveira
Nov 18, 2020·Pest Management Science·Rafael de Almeida BarrosMaria G de Almeida Oliveira
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
Apr 23, 2021·Scientific Reports·Abhijeet P HerwadePankaj K Pawar
Jan 7, 2019·International Journal of Biological Macromolecules·Mauricio V CruzKátia F Fernandes

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.