Box-Behnken design optimization of mucoadhesive chitosan-coated nanoemulsions for rosmarinic acid nasal delivery-In vitro studies

Carbohydrate Polymers
Flávia N S FachelHelder F Teixeira

Abstract

Mucoadhesive chitosan-coated nanoemulsions for rosmarinic acid (RA) nasal delivery were optimized. The optimum ratio between the formulation components that led to minimum droplet size and PDI, and maximal ζ-potential and RA content was obtained using Box-Behnken design (BBD). Optimized conditions were 8.5% oil phase (w/v), 3:10 lecithin to oil phase ratio (w/w), and 0.1% chitosan final concentration (w/v). Physicochemical characterization, mucoadhesion measurement, in vitro release and permeation/retention were performed. Optimized chitosan-coated RA nanoemulsions presented adequate physicochemical characteristics, high mucoadhesive potential, prolonged drug release, and long-lasting permeation time with a higher RA penetration/retention through porcine nasal mucosa. Cell viability and death by necrosis in fibroblasts cells were also evaluated to investigate the formulations safety. Formulations did not induce cytotoxicity following 24 h (3.125-50 μM) or 48 h (3.125-25 μM) of treatments. Overall results demonstrated that optimized chitosan-coated nanoemulsion showed to be a suitable carrier for RA nasal delivery aiming neuroprotective therapies.

Citations

Dec 19, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Beibei HuHeran Li
Nov 27, 2019·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Sucharitha PalagatiBhaskar Reddy Kesavan
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Dec 19, 2020·International Journal of Biological Macromolecules·Sheersha Pramanik, Vaishnavi Sali
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Sep 2, 2020·Colloids and Surfaces. B, Biointerfaces·Patricia Inês BackHelder Ferreira Teixeira
May 17, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Marcela Tavares LuizDelia Rita Tapia-Blácido
Nov 21, 2021·Neurochemical Research·Thuy Trang NguyenGiau Van Vo

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