Box C/D snoRNP Autoregulation by a cis-Acting snoRNA in the NOP56 Pre-mRNA

Molecular Cell
Søren Lykke-AndersenTorben Heick Jensen

Abstract

Box C/D snoRNAs constitute a class of abundant noncoding RNAs that associate with common core proteins to form catalytic snoRNPs. Most of these operate in trans to assist the maturation of rRNAs by guiding and catalyzing the 2'-O-methylation of specific nucleotides. Here, we report that the human intron-hosted box C/D snoRNA snoRD86 acts in cis as a sensor and master switch controlling levels of the limiting snoRNP core protein NOP56, which is important for proper ribosome biogenesis. Our results support a model in which snoRD86 adopts different RNP conformations that dictate the usage of nearby alternative splice donors in the NOP56 pre-mRNA. Excess snoRNP core proteins prevent further production of NOP56 and instead trigger the generation of a cytoplasmic snoRD86-containing NOP56-derived lncRNA via the nonsense-mediated decay pathway. Our findings reveal a feedback mechanism based on RNA structure that controls the precise coordination between box C/D snoRNP core proteins and global snoRNA levels.

Associated Datasets

Citations

May 16, 2019·The EMBO Journal·Katherine E Bohnsack, Markus T Bohnsack
Dec 13, 2019·Nucleic Acids Research·Tomaž BratkovičBoris Rogelj
Feb 25, 2020·The EMBO Journal·Ruth L SealElspeth A Bruford
Apr 9, 2020·Biochemical Society Transactions·Mason A McCoolSusan J Baserga
Feb 6, 2020·Cold Spring Harbor Symposia on Quantitative Biology·Run-Wen YaoLing-Ling Chen
Jul 4, 2019·The EMBO Journal·Katherine E Bohnsack, Markus T Bohnsack
Oct 11, 2020·Biology Open·Douglas M McLaurinMichael D Hebert
Dec 10, 2020·Genome Biology·Simon M HoserAlexander Huettenhofer
Jun 6, 2021·Genome Biology·Étienne Fafard-CoutureMichelle S Scott
Jun 3, 2021·Non-coding RNA·Laeya BaldiniStéphane Labialle
Jun 26, 2021·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Qiankun LiangGuopan Wang
Jun 27, 2021·Nature Communications·Volker BoehmNiels H Gehring
Nov 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Gaëlle J S TalrossJoseph G Gall
Nov 16, 2021·RNA Biology·Daniela Barros-SilvaElena S Martens-Uzunova

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