BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats

Oxidative Medicine and Cellular Longevity
Agata KowalczykAnna Goraca

Abstract

Little is understood of skeletal muscle tissue in terms of oxidative stress and inflammation. Endothelin-1 is an endogenous, vasoconstrictive peptide which can induce overproduction of reactive oxygen species and proinflammatory cytokines. The aim of this study was to evaluate whether BQ123, an endothelin-A receptor antagonist, influences the level of TNF-α, IL-6, SOD-1, HO-1, Nrf2 mRNA, and NF-κB subunit RelA/p65 mRNA in the femoral muscle obtained from endotoxemic rats. Male Wistar rats were divided into 4 groups (n = 6) and received iv (1) saline (control), (2) LPS (15 mg/kg), (3) BQ123 (1 mg/kg), (4) BQ123 (1 mg/kg), and LPS (15 mg/kg, resp.) 30 min later. Injection of LPS led to significant increase in levels of RelA/p65 mRNA, TNF-α, and IL-6, while content of SOD-1, HO-1, and Nrf2 mRNA was unchanged. Administration of BQ123 prior to LPS challenge resulted in a significant reduction in RelA/p65 mRNA, TNF-α, and IL-6 levels, as well as markedly elevated concentrations of SOD-1, HO-1, and Nrf2 mRNA. BQ123 appears to enhance antioxidant defense and prevent production of TNF-α and IL-6 in skeletal muscle of LPS-treated rat. In conclusion, endothelin-A receptor antagonism exerts significant impact on the skeletal muscle favouri...Continue Reading

References

Oct 15, 1992·Biochemical and Biophysical Research Communications·G A VisnerH S Nick
Jun 1, 1994·Chemico-biological Interactions·M MeyerP A Baeuerle
Apr 1, 1997·International Journal of Sports Medicine·H J AppellJ M Soares
Mar 11, 2000·American Journal of Respiratory and Critical Care Medicine·Y FujiiS N Hussain
Mar 18, 2000·Biochemical and Biophysical Research Communications·N DuerrschmidtH Morawietz
Apr 25, 2000·The Journal of Laboratory and Clinical Medicine·J E NelsonC Cardozo
Mar 20, 2001·American Journal of Respiratory and Critical Care Medicine·C TailléJ Boczkowski
Mar 23, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J Roth, G E De Souza
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Esther BarreiroSabah N A Hussain
Mar 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Moshe MarikovskyOri Mahler
Jul 23, 2003·Hypertension·Mona H SedeekJoey P Granger
Aug 9, 2003·Biochemical Pharmacology·Julie A DouthwaiteRoger Corder
Apr 13, 2005·Muscle & Nerve·Charles F Bolton
Jan 4, 2007·Recent Patents on Cardiovascular Drug Discovery·Meri M Hynynen, Raouf A Khalil
Feb 24, 2007·The Journal of Physiology·Chih-Ching YangChau-Fong Chen
Jun 23, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Florian L MullerHolly Van Remmen
Aug 29, 2007·Journal of Intensive Care Medicine·Mariusz PiechotaLucjan Pawlicki
Jan 19, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Zhenyu QinNeal L Weintraub
Jul 22, 2008·The Journal of Surgical Research·Andreas AnderssonHans Hjelmqvist
Dec 4, 2008·The Annals of Thoracic Surgery·Rachael L FordFrancis G Spinale
Sep 1, 2009·Toxicology and Applied Pharmacology·Qiang ZhangMelvin E Andersen
Nov 10, 2009·The European Respiratory Journal·Y ChenK Kubo
Feb 17, 2010·Free Radical Biology & Medicine·Hirotomo KuwaharaTakahiko Shimizu
Aug 14, 2010·Current Drug Targets·William Durante
Dec 29, 2010·Cell Research·Michael J Morgan, Zheng-gang Liu
Mar 1, 2012·Biochimica Et Biophysica Acta·Corey J MillerNamakkal Soorappan Rajasekaran
Feb 7, 2013·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Yu-Chuan TsaiYen-Chin Liu
Apr 6, 2013·Particle and Fibre Toxicology·Fabian HerzogBarbara Rothen-Rutishauser
Apr 11, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Chieh LinTz-Chong Chou
Dec 4, 2013·Current Problems in Pediatric and Adolescent Health Care·Mayer SagyPaul Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme
ELISA
Protein Assay
PCR
Assay
nuclear translocation

Software Mentioned

Sequence Detection System
STATISTICA

Related Concepts

Related Feeds

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here