Brachyspira (Serpulina) hyodysenteriae gyrB mutants and interstrain transfer of coumermycin A(1) resistance

Applied and Environmental Microbiology
T B StantonS B Humphrey

Abstract

To further develop genetic techniques for the enteropathogen Brachyspira hyodysenteriae, the gyrB gene of this spirochete was isolated from a lambdaZAPII library of strain B204 genomic DNA and sequenced. The putative protein encoded by this gene exhibited up to 55% amino acid sequence identity with GyrB proteins of various bacterial species, including other spirochetes. B. hyodysenteriae coumermycin A(1)-resistant (Cn(r)) mutant strains, both spontaneous and UV induced, were isolated by plating B204 cells onto Trypticase soy blood agar plates containing 0.5 microg of coumermycin A(1)/ml. The coumermycin A(1) MICs were 25 to 100 microg/ml for the resistant strains and 0.1 to 0.25 microg/ml for strain B204. Four Cn(r) strains had single nucleotide changes in their gyrB genes, corresponding to GyrB amino acid changes of Gly(78) to Ser (two strains), Gly(78) to Cys, and Thr(166) to Ala. When Cn(r) strain 435A (Gly(78) to Ser) and Cm(r) Km(r) strain SH (DeltaflaA1::cat Deltanox::kan) were cultured together in brain heart infusion broth containing 10% (vol/vol) heat-treated (56 degrees C, 30 min) calf serum, cells resistant to chloramphenicol, coumermycin A(1), and kanamycin could be isolated from the cocultures after overnight incub...Continue Reading

References

Mar 1, 1979·Antimicrobial Agents and Chemotherapy·K KitaiA Arakawa
Jul 8, 1978·The Veterinary Record·A E RitchieJ M Kinyon
Feb 1, 1977·Infection and Immunity·J M KinyonR D Glock
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·I del CastilloF Moreno
Jan 1, 1991·Journal of Bacteriology·M L Holmes, M L Dyall-Smith
Feb 1, 1987·Infection and Immunity·T B Stanton, C P Cornell
Jul 1, 1994·Journal of Clinical Microbiology·R FrothinghamK H Wilson
Feb 1, 1994·Journal of Bacteriology·R L Zuerner, T B Stanton
May 1, 1994·Journal of Bacteriology·D S SamuelsC F Garon
Aug 1, 1993·Molecular Microbiology·A Maxwell
Apr 25, 1993·Nucleic Acids Research·A L Springer, M B Schmid
Jan 1, 1996·International Journal of Systematic Bacteriology·D J TrottD J Hampson
Dec 1, 1995·FEMS Microbiology Letters·S B HumphreyN S Jensen
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·M StiegerH Gmünder
Jan 1, 1996·Annual Review of Genetics·W M Huang
Aug 13, 1998·The Journal of Antimicrobial Chemotherapy·M IpA F Cheng
Oct 31, 1998·Antimicrobial Agents and Chemotherapy·J M BattistiM F Minnick
Mar 27, 1999·Biochemical Society Transactions·A Maxwell
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SartakovaF C Cabello

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Citations

Aug 2, 2003·FEMS Microbiology Letters·Thad B StantonRichard L Zuerner
Jun 12, 2012·Nature Reviews. Microbiology·Andrew S LangJ Thomas Beatty
Aug 20, 2005·Journal of Bacteriology·Eric G MatsonThad B Stanton
Mar 1, 2016·Frontiers in Microbiology·Christian J H von WintersdorffPetra F G Wolffs
Nov 14, 2002·Annual Review of Genetics·Nyles W Charon, Stuart F Goldstein
Aug 9, 2017·Annual Review of Virology·Andrew S LangJ Thomas Beatty

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